[Histonet] Re: Histonet Digest, Vol 114, Issue 14
Naveedafahim
naveedafahim <@t> yahoo.ca
Wed May 15 17:13:15 CDT 2013
Hi Eleanora
We do trichrome on muscle but ph of solution we keep at 3.4 and first hematoxylin then trichrome for 10 minutes and then wash in water 2-3 times and then two changes of 95 percent alcohol then DCM
Naveeda
On 2013-05-15, at 1:02 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. RE: Lysol IC for Ventana equipmetn cleaner (Tom McNemar)
> 2. Re: [IHCRG] p40 (Richard Cartun)
> 3. RE: Gross Room cleaner (Sarah Dysart)
> 4. RE: Re: [IHCRG] p40 (Patsy Ruegg)
> 5. Bone marrow aspirate iron stain (Renz, Jason)
> 6. Gomori Trichrome Staining (Eleonora Guadagnin)
> 7. Re: Gross Room cleaner (Paula Sicurello)
> 8. RE: Bone marrow aspirate iron stain (Tony Henwood (SCHN))
> 9. RE: Lysol IC for Ventana equipmetn cleaner (Sebree Linda A)
> 10. HPV In situ hybridization protocol (FISH or CISH) (Mesru T)
> 11. Mohs histology hourly rate (rook570 <@t> comcast.net)
> 12. Re: Mohs histology hourly rate (Will Chappell)
> 13. Re: Gomori Trichrome Staining (Massimo)
> 14. Re: Mohs histology hourly rate (Jaclyn Pitts)
> 15. Re: Mohs histology hourly rate (Will Chappell)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 14 May 2013 13:15:20 -0400
> From: Tom McNemar <TMcNemar <@t> lmhealth.org>
> Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner
> To: 'Amber McKenzie' <amber.mckenzie <@t> gastrodocs.net>,
> "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <E9A90E28259D2F4E84308C5E8EA8F7B4014717F5E8CB <@t> lmhs-exchange>
> Content-Type: text/plain; charset="us-ascii"
>
> We use it but we only make it up 1 gallon at a time. We use 20ml to 1 gallon of distilled per Ventana.
>
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcnemar <@t> lmhealth.org
> www.LMHealth.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
> Sent: Tuesday, May 14, 2013 12:33 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner
>
>
> I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks!
>
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>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 14 May 2013 13:33:21 -0400
> From: Richard Cartun <Rcartun <@t> harthosp.org>
> Subject: [Histonet] Re: [IHCRG] p40
> To: "'ihcrg <@t> googlegroups.com'" <ihcrg <@t> googlegroups.com>,
> "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>, Jean Taylor
> <jtaylor <@t> meriter.com>
> Message-ID: <51923D21020000770003A980 <@t> gwmail3.harthosp.org>
> Content-Type: text/plain; charset="utf-8"
>
> I have been very impressed with BioCare's "p40" monoclonal predilute.
> We are able to use it a 1:5 dilution on our Bond Max platform.
> Excellent immunoreactivity so far!
>
> Richard
>
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 5/10/2013 1:53 PM >>>
>
> Hi everyone,
>
> I’m looking into ordering the p40 antibody for one of our pathologists.
> He wants to use it to help distinguish between adenocarcinoma and
> squamous cell ca in lung. I’m wondering what labs are using, the
> monoclonal or polyclonal antibody, and where you purchase it from. I
> haven’t found that a lot of companies that carry it. Any info would be
> helpful.
>
> Thanks,
>
> Jean Taylor, HT(ASCP)QIHC
> IHC Tech
> Meriter Health Services
> Madison, WI
>
> --
> --
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> ------------------------------
>
> Message: 3
> Date: Tue, 14 May 2013 17:39:05 +0000
> From: Sarah Dysart <sdysart <@t> mirnarx.com>
> Subject: [Histonet] RE: Gross Room cleaner
> To: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>,
> "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <f6f2daaf10c04c01899a85dc1463d283 <@t> BLUPR07MB001.namprd07.prod.outlook.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Bleach =)
> Just make sure to rinse after cleaning or they will rust
>
> Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
> Histotechnologist
> Mirna Therapeutics
> 2150 Woodward Street
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
> Sent: Tuesday, May 14, 2013 11:29 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Gross Room cleaner
>
>
> What do you guys use to clean your Gross Room utensils? I bought Lem'in Lift from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks!
>
> _______________________________________________
> Histonet mailing list
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>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 14 May 2013 12:38:38 -0600
> From: Patsy Ruegg <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] Re: [IHCRG] p40
> To: Richard Cartun <rcartun <@t> harthosp.org>, ihcrg ihcrg
> <ihcrg <@t> googlegroups.com>, "Histonet <@t> Lists. Edu"
> <histonet <@t> lists.utsouthwestern.edu>, jean taylor <jtaylor <@t> meriter.com>
> Message-ID: <BAY178-W11E21482DA5D15F02AAEA8D8A10 <@t> phx.gbl>
> Content-Type: text/plain; charset="Windows-1252"
>
> Richard now the ab companies are really going to hate you advocating diluting predilute's further isn't that a sacrilege, what are we going to do with you, no negs and now diluting predilutes. I am just kidding I do it all the time myself.
> Cheers, Patsy
>
> Date: Tue, 14 May 2013 13:33:21 -0400
> From: Rcartun <@t> harthosp.org
> To: ihcrg <@t> googlegroups.com; histonet <@t> lists.utsouthwestern.edu; jtaylor <@t> meriter.com
> CC:
> Subject: [Histonet] Re: [IHCRG] p40
>
> I have been very impressed with BioCare's "p40" monoclonal predilute.
> We are able to use it a 1:5 dilution on our Bond Max platform.
> Excellent immunoreactivity so far!
>
> Richard
>
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596 Office
> (860) 545-2204 Fax
>
>
>>>> "Taylor, Jean" <jtaylor <@t> meriter.com> 5/10/2013 1:53 PM >>>
>
> Hi everyone,
>
> I’m looking into ordering the p40 antibody for one of our pathologists.
> He wants to use it to help distinguish between adenocarcinoma and
> squamous cell ca in lung. I’m wondering what labs are using, the
> monoclonal or polyclonal antibody, and where you purchase it from. I
> haven’t found that a lot of companies that carry it. Any info would be
> helpful.
>
> Thanks,
>
> Jean Taylor, HT(ASCP)QIHC
> IHC Tech
> Meriter Health Services
> Madison, WI
>
> --
> --
> You received this message because you are subscribed to the Google
> Groups "ihcrg" group. The IHC Resource Group is a standing committee
> within the National Society for Histotechnology.
>
> To post to this group, send email to ihcrg <@t> googlegroups.com
> To unsubscribe from this group, send email to
> ihcrg+unsubscribe <@t> googlegroups.com
> For more options, visit this group at
> http://groups.google.com/group/ihcrg?hl=en
>
> To contact the National Society for Histotechnology, email:
> histo <@t> nsh.org or call 443.535.4060.
>
> ---
> You received this message because you are subscribed to the Google
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> ------------------------------
>
> Message: 5
> Date: Tue, 14 May 2013 14:06:55 -0500
> From: "Renz, Jason" <jkrenz <@t> primecare.org>
> Subject: [Histonet] Bone marrow aspirate iron stain
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1E0E2B14C709174B8AC2BE0AE7F76833A2F451E3D7 <@t> EXCHANGE2K7.staprimecare.org>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions?
> Jason Renz
> HT(ASCP)/lab safety officer
> St. Alexius Medical Center
> 900 East Broadway
> Bismarck ND, 58506
> (701)530-6733
>
> ________________________________
> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 14 May 2013 15:33:41 -0400
> From: Eleonora Guadagnin <guadagnin.eleonora <@t> gmail.com>
> Subject: [Histonet] Gomori Trichrome Staining
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAKFULy+wVZLXoxLPBdquEc8Rq7pbxd-Q10TtSu9GV_4UimA3QQ <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello everybody,
> I am performing the Gomori Trichrome staining on frozen muscle sections
> (10um) but I have couple of issues:
> 1. The green staining is TOO dark;
> 2. When the green is not too dark, all my muscle fibers are red.
>
> I checked the pH of the Gomori solution, is 2.15.
>
> If anyone has some advice, please!!!!
> Thanks a lot!
>
> Eleonora
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 14 May 2013 18:35:30 -0400
> From: Paula Sicurello <patpxs <@t> gmail.com>
> Subject: Re: [Histonet] Gross Room cleaner
> To: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Cc: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <CAPSjddaaREpPsrbZysAATAHeWzW7xdr58-i3ekASg7=LfNbGGA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dispatch works well too.
>
> Paula
> --
> Paula Sicurello, HTL (ASCP)
> Supervisor, Clinical Electron Microscopy Laboratory
> Duke University Health System
> Rm.#251M, Duke South, Green Zone
> Durham, North Carolina 27710
> P: 919.684.2091
>
> On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie <
> amber.mckenzie <@t> gastrodocs.net> wrote:
>
>>
>> What do you guys use to clean your Gross Room utensils? I bought Lem'in
>> Lift from Mantek in the past to soak the rulers, scissors, tweezers,
>> etc...Thanks!
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 14 May 2013 23:01:06 +0000
> From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
> Subject: [Histonet] RE: Bone marrow aspirate iron stain
> To: "'Renz, Jason'" <jkrenz <@t> primecare.org>,
> "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <6D6BD1DE8A5571489398B392A38A71579D25BA02 <@t> xmdb04.nch.kids>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Jason,
> I am not familiar with the stainer but is the procedure starting from water (not dewaxing & re-hydration as is used for paraffin sections)?
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Renz, Jason
> Sent: Wednesday, 15 May 2013 5:07 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Bone marrow aspirate iron stain
>
> Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions?
> Jason Renz
> HT(ASCP)/lab safety officer
> St. Alexius Medical Center
> 900 East Broadway
> Bismarck ND, 58506
> (701)530-6733
>
> ________________________________
> This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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>
> ------------------------------
>
> Message: 9
> Date: Tue, 14 May 2013 23:24:04 +0000
> From: Sebree Linda A <LSebree <@t> uwhealth.org>
> Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner
> To: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>,
> "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <77DD817201982748BC67D7960F2F76AF04943E <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> The dilution is listed on the
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