[Histonet] RE: Bone marrow aspirate iron stain

Tony Henwood (SCHN) tony.henwood <@t> health.nsw.gov.au
Tue May 14 18:01:06 CDT 2013

Hi Jason,
I am not familiar with the stainer but is the procedure starting from water (not dewaxing & re-hydration as is used for paraffin sections)?

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Renz, Jason
Sent: Wednesday, 15 May 2013 5:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone marrow aspirate iron stain

Hello, We are currently having problems with our bone marrow aspirate slides for iron stains which we run on the Ventana nexes special stainer. The RBCs look crenated or damaged. We currently fix them 5 minutes in methanol then air dry before running them on the stainer. Any suggestions?
Jason Renz
HT(ASCP)/lab safety officer
St. Alexius Medical Center
900 East Broadway
Bismarck ND, 58506

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