[Histonet] Formula 83: xylene substitute

Kiranjit Grewal kiran_g <@t> sbcglobal.net
Tue May 14 11:31:58 CDT 2013

Thanks, Linda. 
What about the odor? Does it have strong or irritating odor? How did you staff responded to the change? We would like to know all before trying it out. Currenly, our staff is having issues with Xylene fumes and goal is to limit any fumes or exposure to Xylene.
I would like to hear from others who tried to use it and didn't work for them.

--- On Tue, 5/14/13, Blazek, Linda <lblazek <@t> digestivespecialists.com> wrote:

From: Blazek, Linda <lblazek <@t> digestivespecialists.com>
Subject: RE: [Histonet] Formula 83: xylene substitute
To: "Kiran" <kiran_g <@t> sbcglobal.net>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, May 14, 2013, 7:13 AM

We use Formula 83 on both staining and processing without any problem at all.  You have to watch and either rotate or change your last Formula 83s on the stain line because you can't tell if there is water in it like you can with xylene.  We also use CBG's recycler to recycle Formula 83 without any problem.  
We have not had any problems with our IHC using Formula 83 either.  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kiran
Sent: Tuesday, May 14, 2013 9:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formula 83: xylene substitute

Hello Everyone,

I would like to get some feedback from Formula 83 users. Are you using it only on the stainers or for tissue processing as well. What kind of validation process is required to switch to this substitute for ihc stains? We are interested to use it for both histo and cyto lab.
Any input is much appreciated.

Thank you in advance,

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