[Histonet] RE: Histonet Digest, Vol 114, Issue 10

Barr,Kaye H khbarr <@t> mdanderson.org
Mon May 13 07:32:53 CDT 2013


The University of Texas M.D. Anderson Cancer Center is in need of a part time pathologist assistant.  The hours will be late afternoon, early evening.  If interested, please apply online at www.mdanderson.org, requisition number 9523.

Kaye Barr, HT (ASCP)  | Laboratory Manager  
M. D. Anderson Cancer Center | Pathology/Histology Labs
1515 Holcombe Blvd, Unit 85 | Houston, TX 77030
713.792.5366 office | khbarr <@t> mdanderson.org

 





-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Saturday, May 11, 2013 12:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 114, Issue 10

Send Histonet mailing list submissions to
	histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Full Time Openings for Histotech and Grossing Tech
      (Laurie Colbert)
   2. RE: Vantage and Prisma (WILLIAM DESALVO)
   3. p40 (Taylor, Jean)
   4. GI biopsy H&E staining (Annie T. Walton)
   5. Re: GI biopsy H&E staining (Jennifer MacDonald)
   6. Extra Ventana Reagent (Delray Beach Pathology Kari Simeone)
   7. Movat Pentachrome - Can?t Remove Woodstain Scarlet-Acid
      Fuchsin from Collagen (Krista Sider)
   8. RE: Movat Pentachrome - Can't Remove Woodstain	Scarlet-Acid
      Fuchsin from Collagen (Elizabeth Chlipala)
   9. ground section staining (Yan Liang)
  10. Re: p40 (Akemi Allison)
  11. Recording formalin "out times" (Wanda Shotsberger Gray)
  12. AW: [Histonet] Movat Pentachrome - Can't Remove Woodstain
      Scarlet-Acid Fuchsin from Collagen (Gudrun Lang)
  13. AW: [Histonet] Recording formalin "out times" (Gudrun Lang)


----------------------------------------------------------------------

Message: 1
Date: Fri, 10 May 2013 17:10:59 +0000
From: Laurie Colbert <lcolbert <@t> pathmdlabs.com>
Subject: [Histonet] Full Time Openings for Histotech and Grossing Tech
To: "Histonet Post (histonet <@t> lists.utsouthwestern.edu)"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<12ECD7346266D74691EC2BFC75285E452F2DDD4B <@t> BFL323E10.pathmdlabs.local>
Content-Type: text/plain; charset="us-ascii"

PATH MD has two full time openings for a histotech and a grossing tech.  Both positions require at least two years of experience and the ability to troubleshoot a variety of issues.  The histotechnician shift is from 11:00pm to 7:30 am and entails embedding, cutting, and manual special stains.  The grossing tech shift is 10:30 am to 7:00 pm, Monday-Friday.

PATH MD is a new pathology reference lab located in West Hollywood, CA.  We are rapidly expanding and are looking to add motivated and experienced personnel to our team.  Salary is competitive and full medical, dental, and vision benefits are offered.  If interested, please forward your resume to: lcolbert <@t> pathmdlabs.com<mailto:lcolbert <@t> pathmdlabs.com>


Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab



------------------------------

Message: 2
Date: Fri, 10 May 2013 10:18:50 -0700
From: WILLIAM DESALVO <wdesalvo.cac <@t> outlook.com>
Subject: RE: [Histonet] Vantage and Prisma
To: "JMaslanka <@t> stpetes.org" <jmaslanka <@t> stpetes.org>, histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY151-W26F4709968C76D23CF236082A50 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

I use Vantage and Sakura Prisma. The Sakura Prisma is not interfaced into Vantage. You place a bar-coded label on your H&E slides at microtomy. We scan the slides after staining, case assembly.

William DeSalvo, BS HTL(ASCP)
Production Manager-Anatomic Pathology
Chair, NSH Quality Management Committee
Owner/Consultant, Collaborative Advantage Consulting

 

> To: histonet <@t> lists.utsouthwestern.edu
> From: JMaslanka <@t> stpetes.org
> Date: Fri, 10 May 2013 09:35:12 -0600
> Subject: [Histonet] Vantage and Prisma
> 
> Happy Friday
> Anyone out there using Ventana's Vantage with Sakura's Tissue-Tek Prisma 
> stainer?
> 
> 
> Joe Maslanka BS, CT,HT (ASCP)
> Anatomical Pathology Technical Supervisor
> St Peter's Hospital,MT 59601
> (P)(406) 447-2406
> (F)(406)444-2126 
> 
> Give thanks for ALL things.....
> "Kindness is the language the blind can see & the deaf can hear- Mark 
> Twain
> 
> 
> 
> This electronic mail message contains information which is confidential. 
> If you are not the intended recipient, please be aware that any 
> disclosure, photocopying, distribution or use of the contents of the 
> received information is prohibited. If you have received this e-mail in 
> error, please reply to the sender immediately and permanently delete this 
> message and all copies of it. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  

------------------------------

Message: 3
Date: Fri, 10 May 2013 12:53:45 -0500
From: "Taylor, Jean" <jtaylor <@t> meriter.com>
Subject: [Histonet] p40
To: "'ihcrg <@t> googlegroups.com'" <ihcrg <@t> googlegroups.com>,
	"'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<B9AF5D89FC58AF4CA27505DB7098496315701B9187 <@t> EXVS1.meriter.com>
Content-Type: text/plain; charset="us-ascii"

Hi everyone,

I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful.

Thanks,

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI


------------------------------

Message: 4
Date: Fri, 10 May 2013 11:02:30 -0700
From: "Annie T. Walton" <AWalton <@t> oima.org>
Subject: [Histonet] GI biopsy H&E staining
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<D8DD32C4FF33B4449B74FF84E553377C06B511A0C3 <@t> OIMAMAIL.oima.local>
Content-Type: text/plain; charset="us-ascii"

Hello all,

Our Medical Director would like the goblet cells on our GI biopsies to stain a darker blue. I hand stain using the following protocol:


 *   Xylene, 3 changes                                                                            3 minutes each
 *   100% Alcohol, 2 changes                                                               1 minute each
 *   95% Alcohol                                                                                        1 minute
 *   Tap water                                                                                            rinse until water runs off evenly
 *   Harris Hematoxylin                                                                          5 minutes
 *   Water wash                                                                                        rinse until water runs clear
 *   Clarifier 2                                                                                             5 seconds
 *   Running water                                                                                  30 seconds
 *   Bluing Solution                                                                                  1 minute
 *   Tap water                                                                                            rinse well
 *   95% Alcohol                                                                                        30 seconds
 *   Eosin Y                                                                                                  1 minute
 *   100% Alcohol, 2 changes                                                               30 seconds
 *   100% Alcohol, 2 changes                                                               1 minute each
 *   Xylene, 3 changes                                                                            1 minute each

I have recently increased the hematoxylin time from 4 to 5 minutes and decreased the time in clarifier from 25 to 5 seconds so the hematoxylin doesn't get washed out as much, but it is not good enough. I also switched from 70% alcohol to 95% before eosin and increased the time in eosin from 45 seconds to 1 minute to provide a greater contrast in hopes that that would work, but the goblet cells are still not staining dark enough. Any suggestions would greatly be appreciated!

Thanks!

Annie Walton, HTL (ASCP)
Histology Technician
Overlake Internal Medicine Associates Histology Lab
425-974-9643
awalton <@t> oima.org


________________________________
DISCLAIMER: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this information is strictly prohibited. If you received this message in error, please notify the sender then delete this message.


------------------------------

Message: 5
Date: Fri, 10 May 2013 11:33:19 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] GI biopsy H&E staining
To: "Annie T. Walton" <AWalton <@t> oima.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <F7BC2EC0-E86E-415D-A144-DDA7C1D0E485 <@t> mtsac.edu>
Content-Type: text/plain; charset=US-ASCII


Use a Gill hematoxylin.

Sent from my iPad

On May 10, 2013, at 11:05 AM, "Annie T. Walton" <AWalton <@t> oima.org> wrote:

> Hello all,
>
> Our Medical Director would like the goblet cells on our GI biopsies to
stain a darker blue. I hand stain using the following protocol:
>
>
>  *   Xylene, 3 changes
3 minutes each
>  *   100% Alcohol, 2 changes
1 minute each
>  *   95% Alcohol
1 minute
>  *   Tap water
rinse until water runs off evenly
>  *   Harris Hematoxylin
5 minutes
>  *   Water wash
rinse until water runs clear
>  *   Clarifier 2
5 seconds
>  *   Running water
30 seconds
>  *   Bluing Solution
1 minute
>  *   Tap water
rinse well
>  *   95% Alcohol
30 seconds
>  *   Eosin Y
1 minute
>  *   100% Alcohol, 2 changes
30 seconds
>  *   100% Alcohol, 2 changes
1 minute each
>  *   Xylene, 3 changes
1 minute each
>
> I have recently increased the hematoxylin time from 4 to 5 minutes and
decreased the time in clarifier from 25 to 5 seconds so the hematoxylin
doesn't get washed out as much, but it is not good enough. I also switched
from 70% alcohol to 95% before eosin and increased the time in eosin from
45 seconds to 1 minute to provide a greater contrast in hopes that that
would work, but the goblet cells are still not staining dark enough. Any
suggestions would greatly be appreciated!
>
> Thanks!
>
> Annie Walton, HTL (ASCP)
> Histology Technician
> Overlake Internal Medicine Associates Histology Lab
> 425-974-9643
> awalton <@t> oima.org
>
>
> ________________________________
> DISCLAIMER: This message is confidential, intended only for the named
recipient(s) and may contain information that is privileged or exempt from
disclosure under applicable law. If you are not the intended recipient(s),
you are notified that the dissemination, distribution or copying of this
information is strictly prohibited. If you received this message in error,
please notify the sender then delete this message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 6
Date: Fri, 10 May 2013 14:46:36 -0400
From: "Delray Beach Pathology Kari Simeone" <KSimeone <@t> leavittmgt.com>
Subject: [Histonet] Extra Ventana Reagent
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<D4E802D6142CDB43923A1DE7CE1D3C72114E62 <@t> advdermexchange.leavittmgt.com>
	
Content-Type: text/plain;	charset="iso-8859-1"

Hi everyone. I use the Ventana NexES and when opening a new kit for PAS, I end up tossing the extra (2) Schiff's 2 (22ml each) bottles. Is there anyone out there that can use this stuff? I'd love to help someone instead of just wasting it. I inquired with Ventana and they basically told me that's just how they sell it. Yuck, wasteful. It's already registered so of no use on any other instrument but who knows?? 
 
Kari M Simeone
Histology/Iummunohistochemisrty Specialist Supervisor
Alternate Laboratory Supervisor
Leavitt Management Group
Advanced Dermatology & Cosmetic Surgery
561.819.0857 ext 100
561.819.6517 fax
ksimeone <@t> leavittmgt.com
www.advancedderm.com <http://www.advancedderm.com/> 

The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message.




------------------------------

Message: 7
Date: Fri, 10 May 2013 17:13:23 -0400
From: Krista Sider <krista.sider <@t> utoronto.ca>
Subject: [Histonet] Movat Pentachrome - Can?t Remove Woodstain
	Scarlet-Acid Fuchsin from Collagen
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<CAG4256oSXLfXkpqqiCM6z0OXEp5gV22je5xxrA0W_9n0DCntsA <@t> mail.gmail.com>
Content-Type: text/plain; charset=windows-1252

Hello All,

I have successfully stained porcine and mouse paraffin embedded heart
tissues with Movat?s Pentachrome (MP), using EMS? MP solutions and protocol
(http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with
some optimization on times.

However, now I am working with archival human tissue that has been fixed
for much longer and is much older than my other samples (Human Aorta &
Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin
and stored at RT for ~6 years). I cannot seem to get the Woodstain
Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5%
Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have
tried increasing the Bouin?s initial fixation (max 2 hrs 50*C), diluting
the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1
hr, yet there is always a lot of red still left in the collagen, in some
regions as strong as the muscle (which I am sure are not muscle or dense
cells). I see virtually no change with increased PA or PM time. As I have
aorta in my samples I can?t just leave out the muscle stain.

I would be very grateful for your insights into anything I could try to get
clean collagen and stained muscle in the MP stain. Why might my method not
be working? Is there something I can substitute for the WSAF that might be
appropriate?

Thank you very much for you help,

Krista


------------------------------

Message: 8
Date: Fri, 10 May 2013 15:26:53 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] Movat Pentachrome - Can't Remove Woodstain
	Scarlet-Acid Fuchsin from Collagen
To: Krista Sider <krista.sider <@t> utoronto.ca>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<14E2C6176416974295479C64A11CB9AE016411AC2D2F <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Krista

Not sure if this would help but we mordant in Bouins for 1 hour at 60C, we let the bouins come up to temp (60C) before we put the slides in.  We have not had good success with temps lower than 60C and if we do not warm up the bouins.  We also find that fresh reagents are key in successful results, we make up our reagents, we don't use a kit.  If you don't need the alcian blue portion of the stain present, my suggestion would be to try a modified trichome, - mordant in bouins and in place of the iron hematoxylin use verhoff elastic stain, differenciate and then counterstain with a regular trichrome.  This will turn out really nice and it will demonstrates elastic fibers, collagen and muscle nicely.  We prefer doing this over the Movats.  I have a protocol if you need one.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Krista Sider
Sent: Friday, May 10, 2013 3:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen

Hello All,

I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat's Pentachrome (MP), using EMS' MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times.

However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta & Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin's initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can't just leave out the muscle stain.

I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate?

Thank you very much for you help,

Krista
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 9
Date: Fri, 10 May 2013 16:43:26 -0500
From: Yan Liang <yliang <@t> numirabio.com>
Subject: [Histonet] ground section staining
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<8DC126BD0D840947A161BBF46AB316880355984F6A <@t> DFW1MBX22.mex07a.mlsrvr.com>
	
Content-Type: text/plain; charset="us-ascii"

Hi Histonet,
We are staining plastic bone ground section. However we have trouble on plastic ground section with H&E, Sandersons with acid fuchsin counterstaining and Goldner's Trichrome staining . It would be great appreciated if you can help  or share protocol with us.

Thanks you
Yan
..............................................
Yan Liang   Ph.D.
Senior Director, Histopathology



------------------------------

Message: 10
Date: Fri, 10 May 2013 15:35:42 -0700 (PDT)
From: Akemi Allison <akemiat3377 <@t> yahoo.com>
Subject: Re: [Histonet] p40
To: "Taylor, Jean" <jtaylor <@t> meriter.com>,	"'ihcrg <@t> googlegroups.com'"
	<ihcrg <@t> googlegroups.com>,	"'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1368225342.75607.YahooMailNeo <@t> web140604.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting last weekend.
?
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3377 <@t> yahoo.com




________________________________
 From: "Taylor, Jean" <jtaylor <@t> meriter.com>
To: "'ihcrg <@t> googlegroups.com'" <ihcrg <@t> googlegroups.com>; "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Friday, May 10, 2013 10:53 AM
Subject: [Histonet] p40
 

Hi everyone,

I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't found that a lot of companies that carry it. Any info would be helpful.

Thanks,

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 11
Date: Fri, 10 May 2013 22:15:01 -0400
From: Wanda Shotsberger Gray <wgray19 <@t> sc.rr.com>
Subject: [Histonet] Recording formalin "out times"
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <fu1e3kbge0hj4um132oa8rc8.1368238501714 <@t> email.android.com>
Content-Type: text/plain; charset=utf-8

Anyone out there using CoPath "paper free"? We are trying to use as little paper as possible, and have yet to come up with a good way/place to record the times that breast tissue comes out of formalin. If we use the "comment" line, the poor tech who scans the cassettes into CoPath must enter the time on every case, which can be way too many cases to make this feasible. We have also tried putting the out time as the name of the run, but the pathologists, who want this info in the report, can't easily see this information, and end up calling us for it.
Any help would be appreciated, and vendors: Are you listening? Make this part of your LIS  product, please.
Thank you all,
Wanda Shotsberger Gray
HT/HTL (ASCP)

------------------------------

Message: 12
Date: Sat, 11 May 2013 10:04:21 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Movat Pentachrome - Can't Remove Woodstain
	Scarlet-Acid Fuchsin from Collagen
To: "'Krista Sider'" <krista.sider <@t> utoronto.ca>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <000001ce4e1e$25ca3f80$715ebe80$@gmx.at>
Content-Type: text/plain;	charset="iso-8859-1"

Try to stain first in PTA/PMA solution to impregnate the collagen fibers -
perhaps testing with different times. Then follow up with red stain and the
usual procedure.

We use a stain called SFOG, that first impregnates 2 min with PMA and
afterwards with the mixture of Chromotrop, Acidfuchsin and Anilinblue for 10
min. The longer we do the Polyacid-step the more intensive are the fibres
and less intensive is the cytoplasma.
I think, if after this trial the collagen is still red, that there are
binding-sites in the collagen, that can't be influenced. Maybe acidfuchsin
is here the main partner and a pure solution of Scarlet Red may help.

Gudrun Lang

-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Krista
Sider
Gesendet: Freitag, 10. Mai 2013 23:13
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Movat Pentachrome - Can?t Remove Woodstain Scarlet-Acid
Fuchsin from Collagen

Hello All,

I have successfully stained porcine and mouse paraffin embedded heart
tissues with Movat?s Pentachrome (MP), using EMS? MP solutions and protocol
(http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with
some optimization on times.

However, now I am working with archival human tissue that has been fixed for
much longer and is much older than my other samples (Human Aorta & Aortic
Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and
stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid
Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid
(PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the
Bouin?s initial fixation (max 2 hrs 50*C), diluting the standard WSAF
solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is
always a lot of red still left in the collagen, in some regions as strong as
the muscle (which I am sure are not muscle or dense cells). I see virtually
no change with increased PA or PM time. As I have aorta in my samples I
can?t just leave out the muscle stain.

I would be very grateful for your insights into anything I could try to get
clean collagen and stained muscle in the MP stain. Why might my method not
be working? Is there something I can substitute for the WSAF that might be
appropriate?

Thank you very much for you help,

Krista
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 13
Date: Sat, 11 May 2013 10:09:21 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Recording formalin "out times"
To: "'Wanda Shotsberger Gray'" <wgray19 <@t> sc.rr.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <000101ce4e1e$d8e51400$8aaf3c00$@gmx.at>
Content-Type: text/plain;	charset="utf-8"

I don't know this system, but is there no possibility to record this time directly in the grossing description? 
Gudrun Lang

-----Urspr??ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Wanda Shotsberger Gray
Gesendet: Samstag, 11. Mai 2013 04:15
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Recording formalin "out times"

Anyone out there using CoPath "paper free"? We are trying to use as little paper as possible, and have yet to come up with a good way/place to record the times that breast tissue comes out of formalin. If we use the "comment" line, the poor tech who scans the cassettes into CoPath must enter the time on every case, which can be way too many cases to make this feasible. We have also tried putting the out time as the name of the run, but the pathologists, who want this info in the report, can't easily see this information, and end up calling us for it.
Any help would be appreciated, and vendors: Are you listening? Make this part of your LIS  product, please.
Thank you all,
Wanda Shotsberger Gray
HT/HTL (ASCP)




------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 114, Issue 10
*****************************************



More information about the Histonet mailing list