[Histonet] H pylori and Ventana instruments

Truscott, Tom ttruscot <@t> vetmed.wsu.edu
Wed May 8 10:57:54 CDT 2013

I haven't used HP, but have noticed this stippled effect especially when using 40X on the microscope and looking at slides labeled with an Ventana alk-phos kit. I seem to have solved the problem by making sure no trace of H2O is left on the slides. It helps after washing slides to run them thru a few changes of alcohol then one acetone and then a couple xylene before coverslipping. Ventana suggested the acetone step. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roger Heyna
Sent: Wednesday, May 08, 2013 8:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] H pylori and Ventana instruments

We use Ventana's HP antibody, and we do see some of the small, non-specific globs of stain, but the pathologists are comfortable differentiating between the true and false staining since they are familiar with this artefact.
Roger Heyna

>>> "Rae Staskiewicz" <raestask <@t> grics.net> 5/7/2013 12:56 PM >>>
Hi all,

Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. 


Rae Staskiewicz
UnityPoint Health-Methodist

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