[Histonet] Cresyl echt Violett for Nissl substance
Lee & Peggy Wenk
lpwenk <@t> sbcglobal.net
Tue May 7 19:02:32 CDT 2013
Cresyl Echt Violet (CEV) is the pre-WWI name. After factories in Germany
were bombed, the formulation for this stain was lost (that's the story I
heard in the 70's).
Closest companies since then have made is cresyl violet acetate (CVA),
though for many decades, continued to call it CEV. Only recently (maybe
last 10 years) have companies been calling it CVA, which is confusing to
labs that need to order a 25 gram bottle once every 25 years.
Here's how we make it up:
CRESYL ECHT VIOLET
Cresyl echt violet (Cresyl Violet Acetate) 0.5 g
Sodium acetate (CH3COONa3H2O) 0.18 g
Distilled water 500.0 mL
Acetic acid, concentrated (CH3COOH) 1.5 mL (up to)
Dissolve cresyl echt violet and sodium acetate in distilled water. Slowly
add acetic acid, drop by drop, to solution. Should have a pH of 3.5. If
solution pH is below 3.5, add more sodium acetate. If solution pH is above
3.5, add more acetic acid. Filter. Let stand overnight before using. Store
at room temperature. Stable for months. May be reused until weak.
Stain for 1-2 hours. Differentiate in 2 changes 95% alcohol, until
background cytoplasm is clear (check with microscope). Run up through 2
changes 100% reagent alcohol, 2 changes xylene or substitute, coverslip.
Nissl, RNA and DNA (nuclei) will be purple.
To speed up differentiation, and to pull more out of the DNA/nuclei, add 2
drops of acetic acid conc. to the first 95% alcohol.
It might be CI # 51180, but I don't have that info at home.
Peggy A. Wenk, HTL(ASCP)SLS
3601 W. 13 Mile Rd.
Royal Oak, MI 48073
Opinions expressed by me to not represent my place of employment.
From: Yaskovich, Ruth A (NIH/NIDCR) [E]
Sent: Thursday, May 02, 2013 3:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cresyl echt Violett for Nissl substance
What is everyone using for this stain?
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