[Histonet] staining of immature collagen in lung fibrosis models

[Elizabeth Chlipala]

Abi

You can perform a Picro Sirius red and that (based upon the literature) will distinguish between Collagen I/III.  You can IHC stain for collagen I and collagen III in mouse, or possibly look at fibroblasts or collagen precursors.  We have not done this in lung but we have stained for a wide variety of IHC markers in wound healing models.  Good Luck.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

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1567 Skyway Drive, Unit E
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of abijag
Sent: Monday, May 06, 2013 10:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] staining of immature collagen in lung fibrosis models

Dear Histonetters,

I am looking for hearing from our experienced histonetters regarding the possibility of staining of immature collagen fibres in animal models of lung fibrosis. I didn't find any histochemical method in the literature to stain the immature collagen. I would like to know whether any specific method is available for staining or is there is possible to differentiate them from mature collagen fibres by means of existing methods(for eg. Scirius red or masson's trichrome)



Thanks in advance



Best regards

Abi Jagannath
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