[Histonet] Staining Nissl

mtitford <@t> aol.com mtitford <@t> aol.com
Fri May 3 10:37:08 CDT 2013

Ruth Yaskovich asks about staining Nissl:

Years ago, when I started in histology and there were still dinosaurs on earth, we used cresyl fast violet  for staining Nissl in celloidin sections, using a fancy differentiation  with cresote in alcohol,  or something.

In paraffin sections you can get pretty good results staining in heated  1% toluidine blue or 0.5% thionin and differentiating in 96% alcohol.

There may be some IHC antibody you can use

Michael Titford
USA Pathology
Mobilew AL USA

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