AW: [Histonet] Monitoring of IHC staining trends

Gudrun Lang gu.lang <@t>
Wed May 1 01:22:11 CDT 2013

We use a composed block of normal tissue for very many antibodies as
positive control. Theoretically it should be visible, if the staining gets
weaker or stronger, if you compare the every-day-the same-picture of the
positive control.
The problems are, that the observers change, that some antibodies are
stained rarely, that the staining-characteristics of some antibodies are not
known well....
I think the feedback of the observers is the most important part here, and
the comparison to the on-slide-control.

Gudrun Lang
Ltd. Biomedizinische Analytikerin
Histolabor Akh Linz

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Troutman,
Kenneth A
Gesendet: Dienstag, 30. April 2013 19:28
An: Histonet <@t>
Betreff: [Histonet] Monitoring of IHC staining trends

Hello Histonet,

I have a question for the group at large.  How are labs monitoring drift in
IHC staining over time?

Here's the scenario:  You do lot to lot testing and everything looks fine
until one day your pathologists are telling you that the CAM5.2 is too dark.
Now, you've been looking at these slides every day for the last year and,
sure enough, when you pull out a slide from last year's lot, it is
significantly lighter.  So what do we do about it?  Do we revalidate the
stain?  Does anyone have a mechanism to monitor this better?  What is the
threshold for revalidation?

Feedback from techs as well as any pathologists would be greatly


Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232
ashley.troutman <@t><mailto:ashley.troutman <@t>>
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