[Histonet] Genta vs alcian blue

[Robert Lott]

As Joyce has pointed out below, the Genta is a Modified Steiner (for
visualization of H.P.) then Alcian Blue (for visualization of signet ring
cells and any other metaplasia) and H&E (for visualization of routine
gastric morphology)...

 

J Clin Pathol. 1997 October; 50(10): 867-868. 

PMCID: PMC500272

Early diagnosis of signet ring cell carcinoma of the stomach: role of the
Genta stain.

H M el-Zimaity, K Itani, and D Y Graham

Department of Medicine, Veterans Affairs Medical Center, Houston, Texas,
USA.

Copyright notice <http://www.ncbi.nlm.nih.gov/pmc/about/copyright.html>  

 

Abstract

Signet ring cell carcinoma is a poorly differentiated adenocarcinoma in
which the tumor cells invade singly or in small groups. Early stages of the
disease can be missed easily when using regular hematoxylin and eosin
staining. This is a report of a case in which routine screening of gastric
biopsies with the Genta stain was responsible for rapid identification of
signet ring carcinoma. The patient, a 29 year old woman, had a large portion
of the antrum excised surgically for signet ring cell gastric carcinoma.
Follow up endoscopy six years later showed no evidence of tumor. Twenty six
large cup biopsies were obtained and a "single focus" of signet ring tumor
cells infiltrating the surface mucosa in single file was seen. The diagnosis
was missed on hematoxylin and eosin stain by three senior pathologists but
owing to "the Alcian blue component" of the Genta stain the tumor cells were
recognized easily. Thus, the Genta stain not only facilitates "detection of
Helicobacter pylori" but also allows for "simultaneous visualization of
gastric morphology" as well as "signet ring carcinoma" that can be missed
with conventional stains.

 

 

Best Regards,

Robert L. Lott

 

Robert L. Lott, HTL(ASCP)

rllott <@t> bellsouth.net

205-746-5628

 

 

 

Message: 7

Date: Fri, 29 Mar 2013 21:52:50 +0000

From: "Weems, Joyce K." <Joyce.Weems <@t> emoryhealthcare.org>

Subject: RE: [Histonet] Genta vs alcian blue

To: "'Nadine Abbott'" <dean1252 <@t> yahoo.com>, histonet

      <histonet <@t> lists.utsouthwestern.edu>

Message-ID:

 
<E3A4EBD57A691646BCCED4AA5911A030990162EF <@t> e14mbx10w.Enterprise.emory.net>

      

Content-Type: text/plain; charset="iso-8859-1"

 

We have used the Genta for Helicobacter. It is a modified Steiner - with
Alcian blue at the end - and then Hematoxylin and eosin. It is easy to see
H. pylori as well as the morphology. I don't know how it relates to signet
ring ca though.

 

Joyce Weems

Pathology Manager

678-843-7376 Phone

678-843-7831 Fax

joyce.weems <@t> emoryhealthcare.org

 

 

 

www.saintjosephsatlanta.org

5665 Peachtree Dunwoody Road

Atlanta, GA 30342

 

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-----Original Message-----

From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nadine
Abbott

Sent: Friday, March 29, 2013 4:39 PM

To: histonet

Subject: [Histonet] Genta vs alcian blue

 

Hello Eveyone,

 

My pathologist has asked me to find out what is the difference in the alcian
blue stain vs the genta stain in assisting in the diagnosis of signet ring
cell carcinoma.

Any assistance you can give me would be much appreciated.

 

Thanks so much.

 

Nadine Abbott, HTL

Grande Ronde Hospital

La Grande, Oregon

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