[Histonet] RE: Biocare Decloaker Nx Gen

[Morken, Timothy]

We tried the Decloaking Chamber Plus a couple years ago after using the Decloaking chamber for years. The Decloaking chamber gave us great results, but the record keeping (time, temp, pressure) for four Decloakers was time consuming and irritating. The PLUS model uses a USB stick to record all that so we didn't have to. A great improvement.  But we ended up going with Bond stainers which obviated all that.

However, it was a good upgrade, and the NX looks even better - Pre-programmed, easier to use, and with the USB feature. 

Pressure cookers and waterbaths generally give more consistent results than steamers since there is variation in the heating within a steam chamber so I think it will help you a lot to change over. With the new instruments' automation it should be painless to operate. 

Tim Morken
UCSF Medical Center
Pathology Dept

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ian R Bernard
Sent: Thursday, March 28, 2013 10:44 AM
To: Swartwood, Steven J; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Biocare Decloaker Nx Gen

I would welcome input hear as well since we are looking at buying one as well.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J
Sent: Thursday, March 28, 2013 12:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biocare Decloaker Nx Gen

Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartwood <@t> cshs.org

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