[Histonet] Biocare Decloaker Nx Gen

[Swartwood, Steven J]

Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartwood <@t> cshs.org

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