[Histonet] WT1 overstaining on Benchmark Ultra

[Dessoye, Michael J]

Hello Histonet,
 
I have two strange occurrences that I'm trying to get to the bottom of.
Running WT1 from Cell Marque on a Benchmark Ultra using iView detection
and the recommended protocol.  Two specimens (both of which happen to be
pleural fluid) severely overstained, even leaving the slide with a brown
background stain.  This has only happened twice, and the slides were on
different positions on different staining runs.  They used the same
antibody and detection kit, however other pleural fluid slides with WT1
are fine.  Other slides that run on the same positions also stain ok.  I
am repeating the stains on the same tissues to try to repeat this but in
the meantime has anyone experienced this condition or have any advice?
 
The only time I've seen this condition before was due to a clogged
vortex mixer, but it affected all the slides and is obviously not the
case here.
 
Thanks!
 
Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdessoye <@t> commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
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