[Histonet] RE: polymer detection

[Elizabeth Chlipala]

Margaret

We personally do not use dual link reagents when working on animal tissue.  We have found some issues with mouse polymers and porcine tissue in the past.  I know the dual links make things a bit simpler but we prefer not to use them at all.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry, Margaret
Sent: Thursday, March 21, 2013 2:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] polymer detection

I would like to get your opinion about rabbit/mouse universal polymer detection systems.  Are there any out there that have little or no background when used on canine ,feline, ovine, bovine, porcine, equine or caprine  tissues?  Right now I use separate polymers for rabbit or mouse antibodies.  We use a DAKO autostainer.  I'm just trying to get a feel about what is new to the field of polymers.
Thank you for your replies.

Margaret Perry HT(ASCP)
Veterinary & Biomedical Sciences Department North Campus Drive Box 2175 South Dakota State University Brookings SD 57007
605-688-5638

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