[Histonet] IHC Stainer Dispense volume

[Tiana Baskin]

I currently use a Dako Autostainer and we are dispensing 300uL of all reagents to the slide(100uL per drop zone). I am just curious what others are dispensing and if you've noticed any difference by changing the dispense volume in regards to stain quality. Does anyone adjust for very large tissue sections (that cover the majority of the slide)?

Thanks :)
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