[Histonet] Thawing frozen tissue

[Hobbs, Carl]

I slice away any excess OCT and immediately immerse in Formalin.
Place on a rocker for 2 days.
If you allow to thaw before fixation...you risk losing more proteins.
Unlikely for many proteins but, not worth it, imho.

Occam's Razor rules 



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
SE1 1UL
Tel: 020 78486813
Fax: 020 78486816
020 78486813