[Histonet] Re: Histonet Digest, Vol 112, Issue 17 ihc validation
M. Kap
m.kap.1 <@t> erasmusmc.nl
Sat Mar 16 12:07:53 CDT 2013
Check out www.cqpath.com, nice references from a2z ;-)
Verstuurd vanaf mijn iPad
Op 16 mrt. 2013 om 18:05 heeft "histonet-request <@t> lists.utsouthwestern.edu" <histonet-request <@t> lists.utsouthwestern.edu> het volgende geschreven:
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. RE: IHC validation (Morken, Timothy)
> 2. RE: IHC validation (WILLIAM DESALVO)
> 3. Thawing frozen tissue (Mesru T)
> 4. Freezing Microtome Special Stains (Walter Benton)
> 5. Microtome repair (Bruce Gapinski)
> 6. RE: RE: AFB and negative control (Debra Siena)
> 7. vibratome mice brain sectionning problem (narjes baazaoui)
> 8. Bone processing help (De La Vega Amador, Rodolfo Enrique)
> 9. FW: No Subject (claudia melidona)
> 10. AW: [Histonet] PCR detection for Mycobacteria (Gudrun Lang)
> 11. RE: IHC validation (Troutman, Kenneth A)
> 12. Best Carmine stain (Kiranjit Grewal)
> 13. Re: RE: IHC validation (Kim Donadio)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 15 Mar 2013 17:05:22 +0000
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> Subject: [Histonet] RE: IHC validation
> To: "Laurie Colbert" <lcolbert <@t> pathmdlabs.com>, "Histonet Post
> (histonet <@t> lists.utsouthwestern.edu)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <761E2B5697F795489C8710BCC72141FF0646B1 <@t> ex07.net.ucsf.edu>
> Content-Type: text/plain; charset=us-ascii
>
> Laurie, for first time validation use controls. Don't use patient cases that are done for initial diagnostics. If they are repeats to test the system, that is ok.
>
> The question is, who tested the controls? Ideally you will send a representative sample of your slides to another lab that uses the same or similar reagents to run for parallel testing.
>
> Tim Morken
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
> Sent: Friday, March 15, 2013 9:51 AM
> To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] IHC validation
>
> If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays?
>
> Laurie Colbert, HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 15 Mar 2013 10:24:08 -0700
> From: WILLIAM DESALVO <wdesalvo.cac <@t> outlook.com>
> Subject: RE: [Histonet] IHC validation
> To: Laurie Colbert <lcolbert <@t> pathmdlabs.com>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <BAY002-W100D2CBE8371575E43BC7A982ED0 <@t> phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Laurie, I would use the control slides to validate and set up the antibodies. I good secondary check/control would be to send slides to another lab to check for correlation and confirmation of your protocol performance. Once you get an established process, then you can later check patient samples to the protocol and make adjustments, as necessary.
>
> William DeSalvo, BS HTL(ASCP)
> Production Manager-Anatomic Pathology
> Chair, NSH Quality Management Committee
> Owner/Consultant, Collaborative Advantage Consulting
>
>
>
>> From: lcolbert <@t> pathmdlabs.com
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Fri, 15 Mar 2013 16:51:27 +0000
>> Subject: [Histonet] IHC validation
>>
>> If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays?
>>
>> Laurie Colbert, HT (ASCP)
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 15 Mar 2013 13:35:25 -0400
> From: Mesru T <turkekul <@t> gmail.com>
> Subject: [Histonet] Thawing frozen tissue
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CACf3QnSWjQ0sNRAN1L-ezsVr=Md0j6jqJpmAyzJB80shv6ASOw <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Maria,
>
> Often people freeze the tissues right after dissecting them out of the
> animal because either they are not sure what to do or they do not know
> better.
> Then they bring the frozen tissues kept at -80 C freezer and request
> paraffin embedding.
> I usually prefer to thaw them gradually. I place the frozen tissues in the
> cryostat (in a tightly sealed zip lock bag), which is at -50 C. Then I
> increase the temperature of the cryostat by 5 C every 45 min minutes until
> it is -5 C. Then I prepare ice cold 10% NBF (or 4% PFA) and place the
> tissues in the ice cold fixative and let the tissues fix at room
> temperature for enough time depending on the size and the type of tissue
> (For my tissues I fixed overnight at room temp). After that I process for
> paraffin embedding. The H&E staining comes fine for frozen tissue. And I
> have tried IHC with 4 different antibodies and had excellent results. I
> have tried mouse brain tumor ( pieces like ~1cm3 in volume) , human brain
> tumor ( small pieces like~ 0.5cm3 in volume) and human lymph nodes (pieces
> like ~3mm3 in volume) with good results. You can also re-freeze after
> thawing but you may have poor tissue architecture.
> I would suggest you to do a pilot trial experiment. You can thaw one block
> that you can spare (or you can prepare a new samples just to test thawing)
> and test how it comes out for your particular case. If you have
> satisfactory results you can go ahead and thaw other samples. I have tried
> to section frozen gelatin embedded tissue in the past and it was very
> difficult. Please take images with your phone and send us the photos of the
> defective blocks.
>
> Good luck!
> Mesru
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 15 Mar 2013 13:43:33 -0400
> From: Walter Benton <wbenton <@t> cua.md>
> Subject: [Histonet] Freezing Microtome Special Stains
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Cc: "Denicia A. Moore" <dmoore <@t> cua.md>
> Message-ID:
> <0B8979A204680A42B93A52B486088CD934229102DB <@t> CUAEXH1.GCU-MD.local>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We have an employee/student that is working on the instrumentation portion of her coursework and came across the Clinical Freezing Microtome (mostly replaced by a cryostat) Carson 3rd ed. pg. 58 . She would like to know if anyone has more information on the types of special stains that require free floating sections using this instrument and if anyone has pictures or links to documentation on the instrument.
>
> Thanks
>
> Walter Benton HT(ASCP)QIHC
> Histology Supervisor
> Chesapeake Urology Associates
> 806 Landmark Drive, Suite 126
> (All Deliveries to Suite 127)
> Glen Burnie, MD 21061
> 443-471-5850 (Direct)
> 410-768-5961 (Lab)
> 410-768-5965 (Fax)
> wbenton <@t> cua.md<mailto:wbenton <@t> cua.md>
>
>
> ________________________________
> CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy.
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 15 Mar 2013 18:29:54 +0000
> From: "Bruce Gapinski" <BGapinski <@t> pathgroup.com>
> Subject: [Histonet] Microtome repair
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <AE98D9CF1513354F83BB0874F39DE86515709D9A <@t> PGNEXCHANGE02.pathgroup.com>
> Content-Type: text/plain; charset=us-ascii
>
> Jeff Myers is the best I've seen in my 35 years. This guy will cut beautiful sections as his final test after repair. He's one of "us".
> (408) 469-0957
>
> Bruce Gapinsk HT (ASCP)
> Chief Histologist
> Marin Medical Laboratories
> PathGroup SF
>
>
> ________________________________
>
> Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 15 Mar 2013 14:10:33 -0500
> From: Debra Siena <DSiena <@t> statlab.com>
> Subject: RE: [Histonet] RE: AFB and negative control
> To: joelle weaver <joelleweaver <@t> hotmail.com>, "Mayer,Toysha N"
> <tnmayer <@t> mdanderson.org>, "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <B7F62D8163C233458A8CEC6920AEE00B32B99FA83F <@t> statsbs>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi All,
>
> According to CLIA Interpretative guidelines, a negative AFB control tissue is to be run each day of testing.
>
> ?493.1256 Standard: Control procedures.
>
> (e)(2) Each day of use (unless otherwise specified in this subpart), test staining materials for intended reactivity to ensure predictable staining characteristics. Control materials for both positive and negative reactivity must be included, as appropriate.
>
> Interpretive Guidelines ?493.1256(e)(2)-(e)(3)
> Acid-fast stains must be checked each day of use for positive and negative reactivity.
>
>
>
> Debbie Siena
> 800.442.3573 ext. 229 | www.statlab.com
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of joelle weaver
> Sent: Thursday, March 14, 2013 3:51 PM
> To: Mayer,Toysha N; 'histonet <@t> lists.utsouthwestern.edu'
> Subject: RE: [Histonet] RE: AFB and negative control
>
> This practice is listed as a QC measure for issues of cross contamination in the ASCP publication "Quality Management in Anatomic Pathology, Nakhleh, R. M.D. I have never had any issues that were persistant enough to warrant this measure myself, but it is one of the suggestions made under the section for use of control tissue/slides.
>
>
>
>
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>> From: TNMayer <@t> mdanderson.org
>> To: histonet <@t> lists.utsouthwestern.edu
>> Date: Thu, 14 Mar 2013 17:52:39 +0000
>> Subject: [Histonet] RE: AFB and negative control
>>
>> While I don't use a negative control for the AFB, I will use distilled water throughout the procedure. Most of the time the water in the waterbath is distilled as well, to rule out contamination there as well. Make sure the waterbath has been disinfected.
>>
>> Toysha N. Mayer, MBA, HT (ASCP)
>> Instructor, Education Coordinator
>> Program in Histotechnology
>> School of Health Professions
>> MD Anderson Cancer Center
>> (713) 563-3481
>> tnmayer <@t> mdanderson.org
>>
>>
>>
>>
>> Date: Thu, 14 Mar 2013 12:42:30 +0000
>> From: Ian R Bernard <ibernard <@t> uab.edu>
>> Subject: [Histonet] AFB and Negative Control
>> To: Lee & Peggy Wenk <lpwenk <@t> sbcglobal.net>, "Tighe, Sean T"
>> <stighe <@t> ufl.edu>, "histonet <@t> lists.utsouthwestern.edu"
>> <histonet <@t> lists.utsouthwestern.edu>
>> Message-ID:
>> <D4F4C602B10B9F45B4E9271AF6380E16181A1114 <@t> UABEXMB1.ad.uab.edu>
>> Content-Type: text/plain; charset="utf-8"
>>
>> The only special stain that I know that requires the use of a negative control is for the AFB. I understand to rule out false positives as the AFB bacteria might exist in tap water. Nevertheless, a good QA practice which we will implement now.
>>
>> Other than Carson, does anyone know of a regulatory or accreditation agency is requiring this as well? Any suggestion on a good control tissue type? Carson recommends uterus. Also if there is a pick up on the negative slide (link to the tap water) will use of distilled water and a repeat procedure fix this?
>>
>> Any thoughts from fellow histonetters?
>>
>> Thanks
>> Ian Bernard
>>
>>
>>
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 15 Mar 2013 16:21:48 -0400
> From: narjes baazaoui <baazaouinarjes <@t> gmail.com>
> Subject: [Histonet] vibratome mice brain sectionning problem
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CAN+Aj0MY0NSsL9WgEPQXrODoPDrqn23cFoLbN2es8TPWz4WRmA <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello,
> I used to section the brains of my mice by microtome I did not have any
> issue. This period I changed to the vibratome I notice that it is cutting
> whole sections at the beginning then it is cutting just parts of the brain
> such as the hippocampus and parietal cortex without the frontal cortex or
> even just the hippocampus without the other structures. I am using cold PBS
> and sharp blade and the angle of the blade is 5 degree. If any body had any
> experience before with the vibratome or encounter this problem please do
> not hesitate to help me.
> Thank you so much.
> Regards.
> Narjes Baazaoui
> --
> Narjes Baazaoui, PhD student
> Institute of Basic research (IBR),
> Laboratory of chemical neuropathology
> 3475995601
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 15 Mar 2013 20:41:23 +0000
> From: "De La Vega Amador, Rodolfo Enrique"
> <RDELAVEGAAMADOR <@t> PARTNERS.ORG>
> Subject: [Histonet] Bone processing help
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <0F62C13F3FBAE541AC2D6AE56E26E85041D30F <@t> PHSX10MB15.partners.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hi everybody,
>
> I am fairly new to all Histotechnology processes. We mainly work in our lab with mineralized bone with implants, so we fix them, dehydrate them, embed them in MMA, cut them, glued them to slides, ground them manually and then stain them. I need help in some parts of the process that need improvement:
>
>
> 1. We use Loctite 4471 on the samples, put them under vacuum, apply them to the slide and more vacuum. There's good results but there are some bubbles that still appear. Suggestions?
> 2. I can't seem to get the yellow/orange on bone with the Van Gieson stain. I've been doinga preheat at 55 ?C, etching, rinse in DI water, Sanderson?s Rapid Bone Stain, running tap water, Van Gieson (commercial from DHM) for 30 seconds to 5 minutes, paper dry and quickly dehydrate with 100% EtOH. What am I doing wrong?
> 3. The stain's been running when I add the glue to cover slip the slides. Especially the green color. Why does this happen?
> 4. Cover slipping is done with cyano acrylate, under suction as before, but bubbles still appear. I've tried placing the cover slip on an angle and wait for the glue to evenly spread, but the bone has just too many pores and air bubbles appear. Please help.
>
> I greatly appreciate any help on the subject.
>
> Best,
>
> Rodolfo De la Vega, MD.
> Research Fellow
> Laboratory for Musculoskeletal Research and Innovation
> Massachusetts General Hospital - Jackson Building 1120
>
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Sat, 16 Mar 2013 01:27:11 -0700 (PDT)
> From: claudia melidona <claudiamel2000 <@t> yahoo.com>
> Subject: [Histonet] FW: No Subject
> To: Bruce Dunlap <GiG <@t> gigsantafe.com>, Gregg Hill
> <gregg <@t> japanesemassage.net>, Staci <gurlie.gurl <@t> verizon.net>, gary
> weidman <gweidman <@t> sbcglobal.net>, histonet request
> <histonet-request <@t> lists.utsouthwestern.edu>, histonet
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1363422431.86984.YahooMailNeo <@t> web162104.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> http://www.christiankeltermann.de/kqbc/kzcgvmemedmii.roplf
>
>
>
> ------------------------------
>
> Message: 10
> Date: Sat, 16 Mar 2013 10:42:33 +0100
> From: "Gudrun Lang" <gu.lang <@t> gmx.at>
> Subject: AW: [Histonet] PCR detection for Mycobacteria
> To: "'Richard Cartun'" <Rcartun <@t> harthosp.org>
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <001501ce222a$9667a9f0$c336fdd0$@gmx.at>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I'm also interested in these assays.
>
> BioProducts in Austria sells a kit vor LightCycler PCR and they state it's
> good for FFPE. But on their website one cannot see the details. It's for 96
> tests.
> I've found a 24-kit from Qiagen, which should work on FFPE. But the assay is
> not validated for it.
>
> Regards
> Gudrun Lang
>
> -----Urspr?ngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Richard
> Cartun
> Gesendet: Freitag, 15. M?rz 2013 17:35
> An: Histonet
> Betreff: [Histonet] PCR detection for Mycobacteria
>
> Is anyone offering clinical PCR detection for Mycobacteria from
> formalin-fixed, paraffin-embedded tissue? Thanks!
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs Assistant Director, Anatomic
> Pathology Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-2204 Fax
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Sat, 16 Mar 2013 14:41:21 +0000
> From: "Troutman, Kenneth A" <Ashley.Troutman <@t> Vanderbilt.Edu>
> Subject: RE: [Histonet] IHC validation
> To: "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Cc: "'lcolbert <@t> pathmdlabs.com'" <lcolbert <@t> pathmdlabs.com>
> Message-ID:
> <D80D248EA7A51F48A622F317D0E643D61C88ADE6 <@t> ITS-HCWNEM108.ds.vanderbilt.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Laurie,
>
> Ideally, you would like to validate both with tissues processed in your laboratory (controls and patient tissue). Purchased TMAs are okay, but they should not be the only thing used.
>
> For the instrument validation, I would definitely use tissue that has been processed in your lab and run the same stain on each instrument (I think we ran serial sections of vimentin) and compare them very closely for any variation (use the same antibody and the same detection kit for each instrument).
>
> My recommendation for new antibody validations is to run a set of normal tissues and tumor tissues (we have about 15 normal tissues that we harvested and created our own sausage block) along with about 20 or so different tumors (also created in sausage blocks) to see how they stain under as many circumstances as possible (especially in the conditions your pathologists are most likely to see).
>
> In addition to that, run a set of known positives and known negatives from your cases. CAP recommends 10 of each, but I believe the final number is at the Medical Director's discretion.
>
> You can also run a representative sample of positives and negatives in your lab and send another set of the same tissues to another lab that has a validated procedure for that antibody and validate it that way.
>
> As far as the detection kit goes, we don't actually "validate" the detection kit per se. We do a lot-to-lot comparison, which involves running tissue from the same block (we created special blocks specifically for detection kits) and run the same antibody (AE1/AE3 or CD3--something we do a LOT of) to compare from lot to lot.
>
> I hope I answered your question.
>
> Regards,
>
> Ashley Troutman BS, HT(ASCP) QIHC
> Immunohistochemistry Supervisor
> Vanderbilt University Histopathology
> 1301 Medical Center Drive TVC 4531
> Nashville, TN 37232
> ashley.troutman <@t> vanderbilt.edu<mailto:ashley.troutman <@t> vanderbilt.edu>
>
> Message: 21
> Date: Fri, 15 Mar 2013 16:51:27 +0000
> From: Laurie Colbert <lcolbert <@t> pathmdlabs.com<mailto:lcolbert <@t> pathmdlabs.com>>
> Subject: [Histonet] IHC validation
> To: "Histonet Post (histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>)"
> <histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>>
> Message-ID:
> <12ECD7346266D74691EC2BFC75285E451CC048EB <@t> BFL323E10.pathmdlabs.local<mailto:12ECD7346266D74691EC2BFC75285E451CC048EB <@t> BFL323E10.pathmdlabs.local>>
> Content-Type: text/plain; charset="us-ascii"
>
> If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays?
>
> Laurie Colbert, HT (ASCP)
>
>
>
> ------------------------------
>
> Message: 12
> Date: Sat, 16 Mar 2013 08:39:06 -0700
> From: Kiranjit Grewal <kiran_g <@t> sbcglobal.net>
> Subject: [Histonet] Best Carmine stain
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <8D2ECF37-6661-478D-886C-566493FDA66A <@t> sbcglobal.net>
> Content-Type: text/plain; charset=us-ascii
>
> Hello,
> Any tips on doing Best Carmine on Frozen sections without water. Can we use mucicarmine solution to pick up glycogen in heart muscle?
> Thank u,
> Kiran
> Sent from my iPhone
>
>
> ------------------------------
>
> Message: 13
> Date: Sat, 16 Mar 2013 09:07:08 -0700 (PDT)
> From: Kim Donadio <one_angel_secret <@t> yahoo.com>
> Subject: Re: [Histonet] RE: IHC validation
> To: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>, Laurie Colbert
> <lcolbert <@t> pathmdlabs.com>, "Histonet Post
> \(histonet <@t> lists.utsouthwestern.edu\)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1363450028.62436.YahooMailNeo <@t> web161601.mail.bf1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I agree about using known controls however sending cases out for comparison?could be a little costly, it is ideal though. One thing i have done is to go back in the records and pull up old cases that have been sent out for IHC and use those to compare not only the test as a case corrolation but also to compare any new controls I might want to continue using. Your pathologist should be able to verify accuracy and sign off on that.??
> ?
> As far as the controls, Id purchase known controls those usually come with a stained example so you can compare that with your new controls and?any old known?cases you may have. Cases( blocks)?that are past thier?legal holding date of course.??
> ?
> Good luck!!
> ?
> Kim D
> ?
> ?
> ?
>
>
> ________________________________
> From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
> To: Laurie Colbert <lcolbert <@t> pathmdlabs.com>; "Histonet Post (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu>
> Sent: Friday, March 15, 2013 1:05 PM
> Subject: [Histonet] RE: IHC validation
>
> Laurie, for first time validation use controls. Don't use patient cases that are done for initial diagnostics. If they are repeats to test the system, that is ok.
>
> The question is, who tested the controls? Ideally you will send a representative sample of your slides to another lab that uses the same or similar reagents to run for parallel testing.
>
> Tim Morken
> Department of Pathology
> UC San Francisco Medical Center
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
> Sent: Friday, March 15, 2013 9:51 AM
> To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] IHC validation
>
> If a lab is just starting up IHC for the first time and has to validate both the IHC stainer and the AB's/detection kit, does the validation have to be done using actual patient cases - or can you use strictly control tissue and purchased microarrays?
>
> Laurie Colbert, HT (ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 112, Issue 17
> *****************************************
More information about the Histonet
mailing list