[Histonet] Re: Histonet Digest, Vol 112, Issue 13

Thu Mar 14 14:31:50 CDT 2013

Have a question to ask the group. One of the Pathologists is complaining 
about anucleate squames on her breast biopsies. Blames the tech for 
fingers being in the water bath, which may or may not happen. If she 
recuts the case it is great and she does not do anything different. what 
could cause  this phenomenon? molds?  It is driving us crazy. HELP

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From:   histonet-request <@t> lists.utsouthwestern.edu
To:     histonet <@t> lists.utsouthwestern.edu
Date:   03/12/2013 10:03 AM
Subject:        Histonet Digest, Vol 112, Issue 13
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu

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Today's Topics:

   1. Re: Fw: news (Kim Donadio)
   2. Detecting monoclonas in mouse lung (Leyva-Grado, Victor)
   3. Reagent containers for VIP 3000 (Vickroy, Jim)

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Message: 1
Date: Mon, 11 Mar 2013 13:47:55 -0400
From: Kim Donadio <one_angel_secret <@t> yahoo.com>
Subject: Re: [Histonet] Fw: news
To: Kim Donadio <one_angel_secret <@t> yahoo.com>
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Disregard something is up with my account. 

Sent from my iPhone

On Mar 11, 2013, at 11:38 AM, Kim Donadio <one_angel_secret <@t> yahoo.com> 
wrote:

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Message: 2
Date: Mon, 11 Mar 2013 19:37:29 +0000
From: "Leyva-Grado, Victor" <victor.leyva-grado <@t> mssm.edu>
Subject: [Histonet] Detecting monoclonas in mouse lung
To: "'histonet <@t> lists.utsouthwestern.edu'"
                 <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<A7D5472945C4E4448FB56D16F814743D071666 <@t> EXCH-2KX-MBXEB2.ExchMail.mssm.edu>

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Dear all,

I have a couple of monoclonal antibodies raised against a respiratory 
virus. I used as treatment for disease and they worked. In trying to 
elucidate some mechanisms, I'm planning to biotinylate the antibody and at 
specific time points collect the lungs and do IHC to determine the 
distribution of the monoclonal. Do you think this will be feasible? I'm 
also planning to use some section for double labeling IF to determine the 
site of interaction antigen-antibody. Do you guys have any reference for 
this?

Thanks a lot,

Victor

Victor H Leyva-Grado DVM, PhD
Postdoctoral Fellow
Microbiology Department
Global Health and Emerging Pathogens Institute
Icahn School of Medicine at Mount Sinai
One Gustave L Levy Place
Box 1124 Annenberg 16-15
New York, NY 10029
Phone 1-212-241-7094
Fax 1-212-534-1684

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Message: 3
Date: Tue, 12 Mar 2013 08:11:09 -0500
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] Reagent containers for VIP 3000
To: "histonet <@t> lists.utsouthwestern.edu"
                 <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
 <BB0B9F1A8373F14FA2974E8CB24BF9CF255C2362 <@t> mmc-mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"

We have two old VIP 3000's and are trying to keep them running. 
Unfortunately finding some parts are getting g very hard.   Can anyone 
steer me to who might have some old reagent containers for the VIP 3000? 
We have one container that started leaking yesterday and I suspect this is 
going to be an ongoing problem because of the age of the instrument. 
Thanks for your assistance.

Jim

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046

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