[Histonet] Fixation

[Geoff]

Rene is correct, formalin fixes slowly so more time is needed. Raising 
the concentration of formaldehyde will not help. Adding a small amount 
of glutaraldehyde (0.25 to 0.5%) will fix more quickly but may have a 
negative effect on immunostaining.

Geoff

On 3/13/2013 6:31 AM, Giulia Zunino wrote:
> I would like to have some informations about fetal brain fixation..
> In general, it is in use the fomalin, but during the cut it is possible to
> see that in the deep layer the formalin doesn't arrive...
> So, Could you give some suggestion to improve this technique?!
>
> Thanks in advance
>
> Best
>
>
>
>
>
> Giulia Zunino, PhD Student
>
> Laboratory of Molecular Neuropathology
> Centre for Integrative Biology (CIBIO), University of Trento
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
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