[Histonet] problem with DAB staining

Nicole Cosenza ncosenza <@t> siumed.edu
Thu Mar 7 11:50:10 CST 2013


Hello all:

I recently did IHC on paraffin embedded slides via the DAB method. Upon 
addition of the DAB, the entire section turned brown. I know this is not 
true staining, as I don't expect a high yield of my protein of interest. 
Any suggestions as to why this happened and what to do about it?



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