[Histonet] Smooth muscle cell actin

[Anatoli Gleiberman]

Patti,
The best antibody (much better than any rabbit, goat or even the same 1A4 clone conjugated with FITC) are:
Mouse monoclonal Anti-alpha-smooth muscle actin Cy3 conjugated, Sigma cat.No.C6198.
Works in dilution 1:500-1:2000 on formaldehyde fixed cell cultures, cryosections (both from fresh frozen and formaldehyde fixed samples, paraffin after formalin etc).
I work with it last 8 years without any problem. They are relatively expensive, but last for long due to high working dilution.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patricia Bourassa
Sent: Thursday, March 07, 2013 8:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Smooth muscle cell actin

Howdy!

Need to attempt immunostaining of smooth muscle cell actin in rat lung.
Any recommendations for reliable SMC primary?

Thanks in advance!  Patti

--
*Patti Bourassa
Senior Scientist
Karos Pharmaceuticals
(203) 535-0540, ext 207*
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