[Histonet] Ethanol denatured with toluene

[Rene J Buesa]

Probably. Toluene is a clearant. Perhaps they are doing that following a very old recipe or some pathologist's preferences, but I do not understand the chemical rational behind that mixture.
rené J.

From: Jackie O'Connor <b427297 <@t> aol.com>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Wednesday, March 6, 2013 4:56 AM
Subject: [Histonet] Ethanol denatured with toluene


I am currently in Germany running a QC inspection of a histology division.  The histo lab uses ethanol denatured with toluene - I have never come across this before.  I have been trying to troubleshoot their H+E stain, and I've been stumped for a long time as to why the sharpness and crispness I'm looking for isn't quite right.  Could the toluene in the ethanol used to make dilutions be the culprit?  I've not been able to find any references to toluene use in US products.  Any input would be greatly appreciated.
Jackie O'
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