[Histonet] teeth sectioning
Jack Ratliff
ratliffjack <@t> hotmail.com
Fri Mar 1 20:11:29 CST 2013
Mes,
This is a very good question and I look forward to answers from individuals that have accomplished this with PMMA and a rotary microtome with tungsten-carbide knives. If you are talking about an undecalcified specimen embedded in PMMA, then I would imagine that the age of the rat could affect the ability to achieve an adequate infiltration of the resin. Again, I look forward to what others have to say about their success by the method you have outlined.
On the other hand, I know you can achieve the micron thickness you desire if you were to use a non-contact femtosecond laser! The machine I am talking about is basically a laser microtome manufactured by Rowiak in Germany and it is officially called the TissueSurgeon. In fact, Dr. Heiko Richter from Germany has accomplished what you ask with human teeth, revealing the full anatomy of the tooth and even with ameloblasts on the enamel surface!
I would be interested to hear more about your project. I will be traveling to Germany one week from today to work with this laser microtome until the end of the month so I could arrange to have laser cut sections made for you if you are interested and unable to make your cuts using PMMA and a rotary microtome. If you would like more information, please feel free to contact me by email reply.
Best Regards,
Jack
Jack L Ratliff
Owner/Histologist, Ratliff Histology Consultants, LLC
Chairman, Hard Tissue Committee - National Society for Histotechnology
On Mar 1, 2013, at 7:03 PM, mesruh turkekul <turkekul <@t> gmail.com> wrote:
> Dear Histonetters,
>
>
> I have one more question. Is it possible to obtain 5-10um thick sections of
> PMMA embedded teeth using regular Leica paraffin microtome (RM2265)
> equipped with disposable tungsten carbide blade?
>
> Thanks,
> Mes
> On Fri, Mar 1, 2013 at 7:40 PM, mesruh turkekul <turkekul <@t> gmail.com> wrote:
>
>> Dear Histonetters,
>>
>>
>> I am studying bone and teeth growth in rat maxilla. I will inject calcein
>> green and would like to fix, embed and sections the rat maxilla.
>> Any suggestions for the best method to fix, embed and section the samples
>> for fluorescnet microscopy?
>>
>> Thank you very much!
>>
>> Mes HTL (ASCP)
>> Memorial Sloan-Kettering Cancer Center
>>
>> On Fri, Mar 1, 2013 at 1:01 PM, <
>> histonet-request <@t> lists.utsouthwestern.edu> wrote:
>>
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>>> Today's Topics:
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>>> 1. FNA Clia Guidelines (PicheGrocki, Jessica)
>>> 2. RE: FNA Clia Guidelines (Horn, Hazel V)
>>> 3. GSH Symposium April 12-14 (Zimmerman, Billie)
>>> 4. FSH abstracts deadline (Jerry Santiago, MSEd, HTL (ASCP) QIHC)
>>> 5. (no subject) (Vikrant Piprode)
>>> 6. The GSH 4oth Anniversary Meeting (David Kemler)
>>> 7. QIHC (Renee H. Workman)
>>>
>>>
>>> ----------------------------------------------------------------------
>>>
>>> Message: 1
>>> Date: Thu, 28 Feb 2013 20:26:30 +0000
>>> From: "PicheGrocki, Jessica" <jpiche-grocki <@t> wtbyhosp.org>
>>> Subject: [Histonet] FNA Clia Guidelines
>>> To: "histonet <@t> lists.utsouthwestern.edu"
>>> <histonet <@t> lists.utsouthwestern.edu>
>>> Message-ID:
>>> <
>>> 631955447A364B45B9458D2905635110655D26B9 <@t> WIN08-MBX-01.wtbyhosp.org>
>>> Content-Type: text/plain; charset="us-ascii"
>>>
>>> Hi All,
>>>
>>> Quick question............what are the Clia requirements for Fine needle
>>> aspirate procedures? Is it considered high complexity testing? And who
>>> prepares the slides when the needle is handed off?
>>>
>>> Thank you,
>>>
>>> Jessica Piche, HT(ASCP)
>>>
>>>
>>>
>>> CONFIDENTIALITY NOTICE: This email and any attachments contain
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>>> ------------------------------
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>>> Message: 2
>>> Date: Thu, 28 Feb 2013 15:05:35 -0600
>>> From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
>>> Subject: [Histonet] RE: FNA Clia Guidelines
>>> To: "'PicheGrocki, Jessica'" <jpiche-grocki <@t> wtbyhosp.org>,
>>> "histonet <@t> lists.utsouthwestern.edu"
>>> <histonet <@t> lists.utsouthwestern.edu>
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>>> <25A4DE08332B19499904459F00AAACB719BE13564F <@t> EVS1.archildrens.org>
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>>> Our pathologists do it all.
>>>
>>> Hazel Horn
>>> Supervisor of Histology/Autopsy/Transcription
>>> Anatomic Pathology
>>> Arkansas Children's Hospital
>>> 1 Children's Way | Slot 820| Little Rock, AR 72202
>>> 501.364.4240 direct | 501.364.1241 fax
>>> hornhv <@t> archildrens.org
>>> archildrens.org
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>>>
>>>
>>>
>>>
>>>
>>> -----Original Message-----
>>> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
>>> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of PicheGrocki,
>>> Jessica
>>> Sent: Thursday, February 28, 2013 2:27 PM
>>> To: histonet <@t> lists.utsouthwestern.edu
>>> Subject: [Histonet] FNA Clia Guidelines
>>>
>>> Hi All,
>>>
>>> Quick question............what are the Clia requirements for Fine needle
>>> aspirate procedures? Is it considered high complexity testing? And who
>>> prepares the slides when the needle is handed off?
>>>
>>> Thank you,
>>>
>>> Jessica Piche, HT(ASCP)
>>>
>>>
>>>
>>> CONFIDENTIALITY NOTICE: This email and any attachments contain
>>> confidential information that is legally privileged. This information is
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>>> ------------------------------
>>>
>>> Message: 3
>>> Date: Thu, 28 Feb 2013 21:41:34 +0000
>>> From: "Zimmerman, Billie" <BZIMMERM <@t> gru.edu>
>>> Subject: [Histonet] GSH Symposium April 12-14
>>> To: "histonet <@t> lists.utsouthwestern.edu"
>>> <histonet <@t> lists.utsouthwestern.edu>
>>> Message-ID:
>>> <
>>> 7B3DEB32E69C034EACB479059C5DE3FF758BF9 <@t> EX-MLB-03.ad.georgiahealth.edu>
>>>
>>> Content-Type: text/plain; charset="us-ascii"
>>>
>>> Don't forget to register for the Georgia Society of Histotechnology
>>> meeting April 12-14.
>>> What a great getaway weekend to earn up to 15 CEU's from the NSH. Join us
>>> for education,
>>> networking, and some great scenery!
>>>
>>> Wanda Simons
>>> President of GSH
>>>
>>> /bz
>>> Augusta State University and Georgia Health Sciences University have
>>> consolidated to become Georgia Regents University. Effective January 9,
>>> 2013, my email address has changed to BZIMMERM <@t> gru.edu. Please update
>>> your address book to reflect this change.
>>>
>>>
>>> ------------------------------
>>>
>>> Message: 4
>>> Date: Thu, 28 Feb 2013 20:51:06 -0500
>>> From: "Jerry Santiago, MSEd, HTL (ASCP) QIHC"
>>> <jerry.santiago <@t> bellsouth.net>
>>> Subject: [Histonet] FSH abstracts deadline
>>> To: histonet <@t> lists.utsouthwestern.edu
>>> Message-ID: <mmcl8cvpbx1vjcacjbxpkjhs.1362102666945 <@t> email.android.com>
>>> Content-Type: text/plain; charset=utf-8
>>>
>>>
>>> Dear histonetters,
>>>
>>> March 1st is the ddeadline to submit any abstracts consideration for the
>>> 2013 Florida Society for Histotechnology meeting. To submit an abstract go
>>> to www.fshgroup.org
>>>
>>> Or email to fsh <@t> fshgroup.org
>>>
>>> Jerry Santiago
>>> FSH
>>>
>>> ------------------------------
>>>
>>> Message: 5
>>> Date: Fri, 1 Mar 2013 00:31:11 -0800 (PST)
>>> From: Vikrant Piprode <vikk_0521 <@t> yahoo.com>
>>> Subject: [Histonet] (no subject)
>>> To: "histonet <@t> lists.utsouthwestern.edu"
>>> <histonet <@t> lists.utsouthwestern.edu>
>>> Message-ID:
>>> <1362126671.40509.YahooMailNeo <@t> web162904.mail.bf1.yahoo.com>
>>> Content-Type: text/plain; charset=iso-8859-1
>>>
>>> hello,
>>> I have tried staining my calvarial bone sections for Tartrate Resistant
>>> Acid Phosphatase but it didnt work out. Can u help me out in this regard.
>>>
>>> Thank you
>>> Vikrant Piprode
>>>
>>> ------------------------------
>>>
>>> Message: 6
>>> Date: Fri, 1 Mar 2013 07:42:31 -0800 (PST)
>>> From: David Kemler <histotalk <@t> yahoo.com>
>>> Subject: [Histonet] The GSH 4oth Anniversary Meeting
>>> To: Fellow HistoNetters <Histonet <@t> Lists.UTSouthwestern.edu>
>>> Message-ID:
>>> <1362152551.97608.YahooMailNeo <@t> web121501.mail.ne1.yahoo.com>
>>> Content-Type: text/plain; charset=iso-8859-1
>>>
>>> Hi Folks - Just a quick reminder that HistoTALK http://www.histotalk.com/has been invited by the GSH board to participate in the festivities again
>>> this year! The GSH meeting is always a great opportunity to help fulfill
>>> your CEU requirements attending workshops by the very best speakers and
>>> presenters in the histology field!
>>>
>>> I'm not presenting this year at the GSH, but I will be there with the
>>> HistoTALK "mobile" studio for guest interviews.
>>>
>>> Jekyll Island is a fantastic place for a state meeting and a great
>>> opportunity to mix and mingle. Be sure to join us for a way-cool time! GSH
>>> members are FUN folks! I know once you go to just one of their
>>> state meetings - you'll be hooked!
>>>
>>> The date is April 12th - 14th, so there's still time to register.
>>>
>>> And again, thanks GSH board for inviting me and HistoTALK to be part
>>> of your 40th Anniversary Celebration!
>>>
>>> Yours,
>>> David
>>> http://www.histotalk.com/
>>>
>>> ------------------------------
>>>
>>> Message: 7
>>> Date: Fri, 1 Mar 2013 16:26:19 +0000
>>> From: "Renee H. Workman" <rhworkman <@t> uro.com>
>>> Subject: [Histonet] QIHC
>>> To: "histonet <@t> lists.utsouthwestern.edu"
>>> <histonet <@t> lists.utsouthwestern.edu>
>>> Message-ID:
>>> <
>>> C9B2D9DC1CB4BF4CAAECB401F244490124F1D345 <@t> BLUPRD0511MB413.namprd05.prod.outlook.com
>>>
>>> Content-Type: text/plain; charset="iso-8859-1"
>>>
>>> I will be taking the QIHC soon has anyone taken it recently. I lost my
>>> QIHC when I changed jobs and want to get
>>>
>>> it back.
>>>
>>>
>>> Renee H. Workman
>>> W: 804-527-1316 | F: 804-270-0917
>>> rhworkman <@t> uro.com<mailto:rhworkman <@t> uro.com> | www.uro.com<
>>> http://www.uro.com/>
>>>
>>>
>>>
>>>
>>>
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>>> End of Histonet Digest, Vol 112, Issue 1
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