[Histonet] HPV ISH Probes

Thu Jun 27 10:22:45 CDT 2013

Me too. I appreciate all of your input and suggestions. I have ordered the Enzo probes to try out.

I will keep you posted.

Cecilia M OBregon, HTL (ASCP)

Memorial Regional Hospital

3501 Johnson Street

Hollywood, FL 33021

954-265-5317

________________________________
From: Susan Foreman [sforeman <@t> labpath.com]
Sent: Thursday, June 27, 2013 11:14 AM
To: Obregon, Cecilia; 'margaret chadwell'; 'Houston, Ronald'; 'joelle weaver'; histonet <@t> lists.utsouthwestern.edu; 'Bitting, Angela K.'
Subject: RE: [Histonet] HPV ISH Probes

Thank you all for your responses regarding HPV-ISH Probes.  I am eagerly awaiting news that folks have this worked up on this platform and optimized.  Kudos to you.

Susan Foreman, HT (ASCP)
KDL Pathology
315 Erin Drive
Knoxville, TN 37919
(865)584-1933

From: Obregon, Cecilia [mailto:CObregon <@t> mhs.net]
Sent: Wednesday, June 26, 2013 1:48 PM
To: margaret chadwell; Houston, Ronald; joelle weaver; Susan Foreman; histonet <@t> lists.utsouthwestern.edu; Bitting, Angela K.
Subject: RE: [Histonet] HPV ISH Probes

What detection are you using?

We only have Ventana instrumentation at our lab.

Thank you

Cecilia M. Obregon, HTL (ASCP)

Memorial Regional Hospital

3501 Johnson Street

Hollywood, FL 33021

954-265-5317

________________________________
From: margaret chadwell [m_chadwell <@t> hotmail.com]
Sent: Wednesday, June 26, 2013 10:49 AM
To: Obregon, Cecilia; Houston, Ronald; joelle weaver; Susan Foreman; histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>; Bitting, Angela K.
Subject: Re: [Histonet] HPV ISH Probes
We have gotten good results with the following probes.

http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview

At first they were not working well but the rep come out for the day and went over them with us and not the results are beautiful and consistent.
Margie Chadwell
Sent from Windows Mail

From: Bitting, Angela K.
Sent: ‎Wednesday‎, ‎June‎ ‎26‎, ‎2013 ‎6‎:‎28‎ ‎AM
To: Obregon, Cecilia, Houston, Ronald, joelle weaver, Susan Foreman, histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>

I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097, Montgomery, TX 77356, 1-800-338-9579

-----Original Message--http://www.panomics.com/products/rna-in-situ-analysis/viewrna-ish-tissue-assay/overview---
From: histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia
Sent: Tuesday, June 25, 2013 4:33 PM
To: Houston, Ronald; joelle weaver; Susan Foreman; histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] HPV ISH Probes

I have personally tried the Dako probes on the Ventana platform but no luck so far.

I have heard about the Enzo probes been succesfully worked up at other institutions. Does anybody know if a 'link antibody' is needed for the Enzo probes?

Greatly appreciate any inputs.

Cecilia M. Obregon, HTL (ASCP)
Memorial Regional Hospital
3501 Johnson Street
Hollywood, FL 33021
Ph: 954-265-5317
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Houston, Ronald [Ronald.Houston <@t> nationwidechildrens.org]
Sent: Tuesday, June 25, 2013 3:41 PM
To: joelle weaver; Susan Foreman; histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] HPV ISH Probes

Many people are working on the Enzo probes just now. Mayo does have the probes up and running on the Bonds, but are keeping tight-lipped about their protocols.
We tried the Dako probes but never could get consistent results.

Ronnie Houston MS HT(ASCP)QIHC

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, June 25, 2013 3:34 PM
To: Susan Foreman; histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] HPV ISH Probes

HPV6, 11, 16 18, 31, 33 & 51 "screening'

Joelle Weaver MAOM, HTL (ASCP) QIHC

> From: sforeman <@t> labpath.com<mailto:sforeman <@t> labpath.com>
> To: histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
> Date: Tue, 25 Jun 2013 14:10:59 -0400
> Subject: [Histonet] HPV ISH Probes
>
> Does anyone have HPV-ISH working?  utilizing a vendor for the probe
> other than Ventana?  Prep Kit?
>
>
>
>
>
> Many Thanks,
>
> Susan Foreman, HT (ASCP)
> (865)584-1933
>
>
>
>
>
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> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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