[Histonet] Mixing Paraffin Brands

[Lucie Guernsey]

Thank you all for your responses! I'm so glad I asked - I didn't realize
that plus was softer than xtra. Now I'm definitely interested in trying to
embed using the xtra since sometimes I feel like our blocks could be
harder. I think I'll follow the majority's advice: infiltrate using
Fisher's Paraplast Plus and embed using McCormick's Paraplast X-tra.

Thanks again!
Lucie

Lucie Guernsey
UCSD
Dept. of Pathology
lguernsey <@t> ucsd.edu



On Wed, Jun 19, 2013 at 1:08 PM, Elizabeth Chlipala <liz <@t> premierlab.com>wrote:

> That's what we do and we do not have any issues with bone, we use
> paraplast to infiltrate and paraplast extra to embed in.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> liz <@t> premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debra Siena [
> DSiena <@t> statlab.com]
> Sent: Wednesday, June 19, 2013 12:29 PM
> To: Lucie Guernsey; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Mixing Paraffin Brands
>
> Hi Lucie,
>
> Paraplast Plus has DMSO and Paraplast Xtra doesn't.  It may be best to
> infiltrate with the Plus which is softer and then embed with the Paraplast
> Xtra which is a bit harder.  I don't usually recommend mixing them
> especially if you only have a few bags.  Thanks
>
> Debbie Siena
> 800.442.3573 ext. 229 | www.statlab.com
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey
> Sent: Wednesday, June 19, 2013 1:01 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Mixing Paraffin Brands
>
> Hi,
>
> Does anyone know if there's a reason why one shouldn't mix different
> brands of paraffin? We normally use Fisherbrand's Paraplast Plus (melting
> point 56 C). We inherited about 8 bags of McCormick Paraplast X-tra
> (melting point
> 52 C). Will the different melting points be a problem?
>
> If we were to use the McCormick paraffin, the only place it may mix with
> the Fisherbrand paraffin is in the blocks themselves (as we refill the
> embedder). But I don't want to compromise the quality of our blocks just to
> not waste the free paraffin.
>
> Or, another option could be that we use the McCormick in the processor and
> the Fisherbrand in the embedder. Could that cause issues in the blocks as
> the tissue would be infiltrated with one brand and embedded in another?
>
> Maybe I'm over-thinking this......
>
> Many thanks!
> Lucie
>
> Lucie Guernsey
> UCSD
> Dept. of Pathology
> lguernsey <@t> ucsd.edu
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>