[Histonet] Mixing Paraffin Brands

[Jack Ratliff]

Pedro,
I have been using a superb method from Bob Skinner for all my decalcified bone work. Basically, I infiltrate with 50% TissuePrep from Fisher Scientific and 50% EM400 from Leica. I then embed in straight EM400. I personally feel that I get good infiltration and I never have had a problem with my bone cutting. (knocking twice on wood....LOL) I might also mention that I decalcify with 5% formic acid and clear after dehydration using methyl salicylate instead of xylenes.
Jack


Jack L RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, Hard Tissue Committee - National Society for Histotechnology


> From: JGarfield <@t> lifecell.com
> To: LouroP <@t> Princeton.Huntingdon.com; rjbuesa <@t> yahoo.com; lguernsey <@t> ucsd.edu; histonet <@t> lists.utsouthwestern.edu
> Date: Wed, 19 Jun 2013 14:17:22 -0500
> Subject: RE: [Histonet] Mixing Paraffin Brands
> CC: 
> 
> Pedro,
> 
> I recommend Paraplast-Xtra.  
> 
> Regards,
> Jackie
> 
> Jacqueline D. Garfield | Manager, Histology
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Louro, Pedro
> Sent: Wednesday, June 19, 2013 2:33 PM
> To: Rene J Buesa; Lucie Guernsey; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Mixing Paraffin Brands
> 
> Just out of curiosity, what paraffin would people out there recommend using for animal bone joints and turbinates?
> We are currently using parapalst plus and was thinking of changing to a "harder paraffin"
> 
> Any thoughts???
> 
> Pedro L.
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Wednesday, June 19, 2013 2:24 PM
> To: Lucie Guernsey; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Mixing Paraffin Brands
> 
> Each paraffin has some additives to improve either its penetration rate or density to section.
> Mixing different melting points (MP) paraffin will result in another paraffin with an intermediate MP and the sectioning will be different.
> Preparing the block with the mixture will probably cause so troubles while sectioning.
> Why don't you just use them separate? There is no good reason to mix them and the two paraffins you mention are of good quality.
> René J.
> 
> From: Lucie Guernsey <lguernsey <@t> ucsd.edu>
> To: histonet <@t> lists.utsouthwestern.edu 
> Sent: Wednesday, June 19, 2013 2:01 PM
> Subject: [Histonet] Mixing Paraffin Brands
> 
> 
> Hi,
> 
> Does anyone know if there's a reason why one shouldn't mix different brands
> of paraffin? We normally use Fisherbrand's Paraplast Plus (melting point 56
> C). We inherited about 8 bags of McCormick Paraplast X-tra (melting point
> 52 C). Will the different melting points be a problem?
> 
> If we were to use the McCormick paraffin, the only place it may mix with
> the Fisherbrand paraffin is in the blocks themselves (as we refill the
> embedder). But I don't want to compromise the quality of our blocks just to
> not waste the free paraffin.
> 
> Or, another option could be that we use the McCormick in the processor and
> the Fisherbrand in the embedder. Could that cause issues in the blocks as
> the tissue would be infiltrated with one brand and embedded in another?
> 
> Maybe I'm over-thinking this......
> 
> Many thanks!
> Lucie
> 
> Lucie Guernsey
> UCSD
> Dept. of Pathology
> lguernsey <@t> ucsd.edu
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