[Histonet] Re: Histonet Digest, Vol 115, Issue 13
Andrea.X.Dejager <@t> kp.org
Andrea.X.Dejager <@t> kp.org
Thu Jun 13 12:25:39 CDT 2013
This message is for Casey Arnold, we never had any problems with our
stainer/coverslipper, I will send you our procedure to try out. Do you dry
slides on the stainer or elsewhere?
Andrea De Jager, H.T. ASCP
Histology Manager, Regional Reference Lab
Kaiser Permanente - Colorado
Phone: 303-404-4152
Fax: 303-404-4161
email: ANDREA.X.DEJAGER <@t> KP.org
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From: histonet-request <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Date: 06/13/2013 11:04 AM
Subject: Histonet Digest, Vol 115, Issue 13
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
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Today's Topics:
1. Tissue Processors (Matthew Roark)
2. Histology Openings (Anna Nolan)
3. Paraffin processing native sheep ACL (Elizabeth Ronan)
4. RE: Shandon Excelsior Tissue Processor (Tony Henwood (SCHN))
5. RE: Shandon Excelsior Tissue Processor (Marcum, Pamela A)
6. Sakura Tissue-Tek Prisma (Casey Arnold)
7. RE: Control Storage (Bitting, Angela K.)
8. Old books (Amber McKenzie)
9. cutting arterial grafts (Megan Elizabeth Flynn)
10. RE: Electron Microscopy protocols (idimitro <@t> mun.ca)
----------------------------------------------------------------------
Message: 1
Date: Wed, 12 Jun 2013 13:18:51 -0500
From: "Matthew Roark" <mroark <@t> sfmc.net>
Subject: [Histonet] Tissue Processors
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000f01ce6799$4a64f2b0$df2ed810$@net>
Content-Type: text/plain; charset="us-ascii"
So in the next couple of months we are getting ready to demo Thermo's STP
420ES and Leica's ASP6025 tissue processor .
Looking for the good, bad, and ugly about them. So if you have one or
have had a demo please share what you've found.
Thanks!!
Matthew Roark- HT/HTL(ASCP)CM
Histology Specialist
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
<mailto:mroark <@t> sfmc.net> mroark <@t> sfmc.net
<http://www.sfmc.net> http://www.sfmc.net
------------------------------
Message: 2
Date: Wed, 12 Jun 2013 15:19:34 -0400
From: "Anna Nolan" <anolan <@t> prometheushealthcare.com>
Subject: [Histonet] Histology Openings
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <00c801ce67a1$c696cbd0$53c46370$@prometheushealthcare.com>
Content-Type: text/plain; charset="us-ascii"
Hi my name is Anna and I work with Prometheus Healthcare doing laboratory
recruitment. Currently I'm looking to fill several histology positions.
Please call or email me if any of these positions are of interest to you.
Portland, ME
-Histology Technologist (3AM-11AM)
NYC
-Grossing Specialist (3rd shift)
-Histology Tech (1st shift)
Suffern, NY
-IHC Histology Tech (1st shift)
-Cutting embedding, staining, and coverslipping. IHC Experience a
plus.
(7AM-4PM)
Anna Nolan
Recruiter
Prometheus Healthcare
Office 301-693-9057
Cell 301-693-8908
Fax 301-368-2478
<http://anolan@prometheushealthcare.com/> anolan
<mailto:chris <@t> prometheushealthcare.com> @prometheushealthcare.com
<http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6>
http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6
<http://www.linkedin.com/profile/view?id=197035050>
<http://www.prometheushealthcare.com/> www.prometheushealthcare.com
------------------------------
Message: 3
Date: Wed, 12 Jun 2013 16:59:52 -0400
From: Elizabeth Ronan <lizronan <@t> umich.edu>
Subject: [Histonet] Paraffin processing native sheep ACL
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <51B8E148.3020102 <@t> umich.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Hello,
I need to paraffin process native sheep anterior cruciate ligament (ACL)
that has been fixed in 10% neutral buffered formalin for 7 days. I was
wondering if anyone with more expertise on this subject could guide me
with the best lengths of times in the alcohols, xylenes, and paraffin to
fix ACL. I tried a 12 hour program but the sections crumbled in the
middle and it appeared that the paraffin had not fully perfused the
ligament.
I have access to the following program, and can alter the lengths of the
steps for as long as desired:
Program:
70%
80%
95%
95%
100%
100%
Xylene
Xylene
Paraffin
Paraffin
Paraffin
Any advice is much appreciated.
Thanks for your time,
Liz
------------------------------
Message: 4
Date: Wed, 12 Jun 2013 23:40:22 +0000
From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
Subject: RE: [Histonet] Shandon Excelsior Tissue Processor
To: "'Velez, Vanessa'" <vvelez <@t> usgs.gov>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6D6BD1DE8A5571489398B392A38A71579D271C66 <@t> xmdb04.nch.kids>
Content-Type: text/plain; charset="us-ascii"
Vanessa,
We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector.
Cost under $1,500 Australian.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Velez,
Vanessa
Sent: Thursday, 13 June 2013 2:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Shandon Excelsior Tissue Processor
Does any one having problems with the Excelsior Tissue Processor power
board repeatedly after power outage? This has happened to me three times
in the last five years even though it has backup batteries.
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Message: 5
Date: Thu, 13 Jun 2013 11:05:48 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: RE: [Histonet] Shandon Excelsior Tissue Processor
To: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>, "'Velez,
Vanessa'" <vvelez <@t> usgs.gov>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<41D3A1AF6FEF0643BDC89E0516A6EA32CA17004C <@t> Mail2Node2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
Since we have some power issues here and 4 Excelsiors (no problems with
them on this issue) we have UPS units on all of our computerized
equipment. We did this due to issues in other areas of the lab and
decided it was safer than taking a chance and being down. We bought
Powervar and I think the suggested units were around $2,000 to $3,000 each
with one unit serving two processors. Other sites in Little Rock who have
some of the same power issues have had problems.
Pam Marcum
UAMS
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Tony Henwood
(SCHN) [tony.henwood <@t> health.nsw.gov.au]
Sent: Wednesday, June 12, 2013 6:40 PM
To: 'Velez, Vanessa'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Shandon Excelsior Tissue Processor
Vanessa,
We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector.
Cost under $1,500 Australian.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Velez,
Vanessa
Sent: Thursday, 13 June 2013 2:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Shandon Excelsior Tissue Processor
Does any one having problems with the Excelsior Tissue Processor power
board repeatedly after power outage? This has happened to me three times
in the last five years even though it has backup batteries.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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This note also confirms that this email message has been virus scanned and
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------------------------------
Message: 6
Date: Thu, 13 Jun 2013 08:15:23 -0500
From: Casey Arnold <carnold <@t> ourlab.net>
Subject: [Histonet] Sakura Tissue-Tek Prisma
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAFDW3OgGzHvR7hrd32P27-mYcWWUsn-Z7CXySK2z0iBaROmQ6A <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
We have recently started using a Sakura Tissue-tek Prisma that we
inherited
from our sister lab. While running some validation testing I have noticed
that the staining is very inconsistent. The tissue will stain from light
to
dark within the same rack and sometimes within the same cut (slide). I
have
reset the mix, speed, etc, to factory settings. I have tried increasing my
hematoxylin, and bluing times. I have changed water filters, decreased
water rinse times, tried different types of tissue, all to no avail. Has
anyone had this problem or have any suggestions on how to correct it? I
have tried everything the Sakura tech line has recommended and am still at
a loss.
--
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Thank you
*Casey Arnold
*
*Histology Supervisor**
OURLabs / OPKO Diagnostics, LLC*
1450 Elm Hill Pike
Nashville, TN 37210
Direct: 615-345-4582
Office: 615-874-0410
Fax: 615-345-4595
------------------------------
Message: 7
Date: Thu, 13 Jun 2013 14:21:16 +0000
From: "Bitting, Angela K." <akbitting <@t> geisinger.edu>
Subject: [Histonet] RE: Control Storage
To: Elizabeth Chlipala <liz <@t> premierlab.com>, "Herring, Colleen"
<Colleen_Herring <@t> bshsi.org>,
"Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77F52EFAB8B1694B885E277C48FCD0F647C7298D <@t> GHSEXMBX4W8K1V.geisinger.edu>
Content-Type: text/plain; charset="us-ascii"
Something to remember is that humidity is the enemy too. If you are
storing your slides in a refrigerator make sure that the humidity is well
controlled.
We found out the hard way.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Wednesday, June 12, 2013 11:56 AM
To: Herring, Colleen; Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Control Storage
We store our blocks at room temp, our IHC slides are stored in the fridge.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592 Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz <@t> premierlab.com
Ship to address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Herring, Colleen
[Colleen_Herring <@t> bshsi.org]
Sent: Wednesday, June 12, 2013 9:48 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Control Storage
Does anyone have any ideas or suggestions about the storage of blocks and
precut slides for immunos?
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Message: 8
Date: Thu, 13 Jun 2013 14:22:10 +0000
From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] Old books
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5A33C952BB67F4468AF1F36D739212BCBD6ADF63 <@t> JERRY.Gia.com>
Content-Type: text/plain; charset="us-ascii"
I'm cleaning house :) Anyone interested in buying these old books?
1. A color Atlas of Histology by Dennis Strete, ISBN 0-06-501084-1
2. Human Histology, 2nd edition, by Alan Stevens and James Lowe, ISBN
0-7234-2485-3
3. Immunolgy, 4th edition, by Roitt, Brostoff, Male ISBN 0-7234-2178-1
4. Atlas of Histology with Funcitonal correlations, by Victor P.
Erodschenko ISBN 0-583-2818-9
5. Histotechnology, A Self Instructional Text, 2nd edition, by Freida
Carson (I'm interested in purchasing the 3rd edition).
------------------------------
Message: 9
Date: Thu, 13 Jun 2013 15:11:12 +0000
From: Megan Elizabeth Flynn <megan-flynn <@t> northwestern.edu>
Subject: [Histonet] cutting arterial grafts
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5748287483CD8A48BC4A27D05908D4E215E72FFC <@t> CHCSPMBX3.ads.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Hi all,
I am in a research lab and I am cutting rat arterial grafts made with
ePTFE. I'm cutting at 30 degrees at 5 microns. The lower edge of the
graft seems to be lifting up from the adventitia and folding over. I have
tried playing with the temperature, but none of it seems to make a
difference. Can anyone help?
Megan
------------------------------
Message: 10
Date: Thu, 13 Jun 2013 15:55:23 +0000
From: <idimitro <@t> mun.ca>
Subject: RE: [Histonet] Electron Microscopy protocols
To: <joel.haas <@t> gladstone.ucsf.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14C3108E8B98EF43B3EDAE58336700341FA60F <@t> exchange.med.mun.ca>
Content-Type: text/plain; charset="us-ascii"
Hi Joel,
Lipids are fixed only by OsO4, so you need to use it as a post-fixative.
As a fixative in our lab we use Karnovsky fixative, then we use osmium
tetroxide as a post-fixative/fixative for the lipids for 15 min. Then we
wash in Na-cacodylate buffer for 5 min, and then dehydrate, starting with
two changes of 70% ethanol ( for 10min.) and then we go to two changes of
95% and then absolute ethanol, followed by two changes of abs. acetone,
then 50:50 acetone:resin and 2 changes of resin, then embedding and
polymerization at the right temperature.
If your lipids are fixed properly they will be round droplets with
characteristic appearance. OsO4 will blacken the tissue.
We always have excellent results with this protocol.
Hope that was helpful,
Iliana dimitrova,
Memorial university of Newfoundland,
Canada
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joel Haas
Sent: June-05-13 3:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Electron Microscopy protocols
Hello all,
I am working on developing an electron microscopy protocol for looking
at lipid droplets in cultured cells. We have used thin section TEM
previously and found that the morphology of the droplets is often deformed
(should be spherical, shows up as egg shaped or lumpy). If possible we
would like to avoid these types of preservation artifacts. Does anyone
have suggestions for adapting the protocol to better preserve these types
of structures--a triglyceride lipid core surrounded by a phospholipid
monolayer?
Thanks in advance,
Joel
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