[Histonet] RE: Her-2 antibody

[Sue Hunter]

We have contacted Leica several times, had the machine calibrated/aligned many times, and that is what we have been told.  It seems to hold true as with only one label we have no problems.  Also, after the upgrade, we saw the problem a lot when we only ran one or two slides on a tray.  The magic number seems to be 4 slides to get acceptable staining, but Leica suggests we load it up.   We had never had a problem until the upgrade in software.
Sue

-----Original Message-----
From: Houston, Ronald [mailto:Ronald.Houston <@t> nationwidechildrens.org] 
Sent: Wednesday, June 12, 2013 10:59 AM
To: Sue Hunter; Robert Fauck [CCDHB]; histonet <@t> lists.utsouthwestern.edu
Subject: RE: Her-2 antibody

Never had any such problems with either the Max or the Bond IIIs in the 6+ years we have had them(no matter how many labels are on the slide - we have had three labels on a slide with no problems at all), unless of course your tissue extends to the rim of the covertile - then what would you expect?
You certainly do not need to have all 10 spaces filled on the tray. 
Suggest you contact Leica service as it seems as though your machine needs calibrated and realigned

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Wednesday, June 12, 2013 10:42 AM
To: Robert Fauck [CCDHB]; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Her-2 antibody

We have a Bond Max and run mainly ER/PR and ISH stains on that.  After Leica loaded in a new version of software in October we started having problems like that too.  Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight.  We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining.  Even a regular slide label under the Leica label will cause a problem.  We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification.  Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here.  As they say, The Enemy of Good is Better.  Wish Leica had not made it better!  It was working very nicely before.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB]
Sent: Tuesday, June 11, 2013 1:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Her-2 antibody

Hi All
 
We here in wellington Hospital New Zealand having some problem with the
Her-2 ( Lieca, NCL-L-CBE-356 )
 
Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative!
 
Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III.
 
Much appreciated for any suggestions, also from Leica Technical specialists.
 
Regards,
Robert Fauck
Wellington hospital, NZ


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