[Histonet] RE: Her-2 antibody

[Sue Hunter]

We have a Bond Max and run mainly ER/PR and ISH stains on that.  After Leica loaded in a new version of software in October we started having problems like that too.  Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight.  We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining.  Even a regular slide label under the Leica label will cause a problem.  We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification.  Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here.  As they say, The Enemy of Good is Better.  Wish Leica had not made it better!  It was working very nicely before.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB]
Sent: Tuesday, June 11, 2013 1:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Her-2 antibody

Hi All
 
We here in wellington Hospital New Zealand having some problem with the
Her-2 ( Lieca, NCL-L-CBE-356 )
 
Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative!
 
Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III.
 
Much appreciated for any suggestions, also from Leica Technical specialists.
 
Regards,
Robert Fauck
Wellington hospital, NZ


This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board.

http://www.ccdhb.org.nz

(1C_S1)
 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet