[Histonet] RE: Her-2 antibody
SHUNTER <@t> beaumont.edu
Wed Jun 12 09:41:41 CDT 2013
We have a Bond Max and run mainly ER/PR and ISH stains on that. After Leica loaded in a new version of software in October we started having problems like that too. Seems you have to have all 10 spots on the tray filled with a slide - doesn't have to be programed, just a slide and cover tile for the weight. We also were doing a workaround with a Vantage label under the Leica label and found out that the two label thickness interferes with the movement of the cover tile (sometimes...but not always), which was affecting our staining. Even a regular slide label under the Leica label will cause a problem. We finally have gone to entering a case, printing the labels but not putting them on the slides, cutting the sections and then putting the labels on the slide for positive patient identification. Not ideal, and I worry a lot about case mix ups, but that is what is working to get consistent staining here. As they say, The Enemy of Good is Better. Wish Leica had not made it better! It was working very nicely before.
Sue Hunter, Supervisor
Beaumont Health System
Royal Oak MI
shunter <@t> beaumont.edu
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Fauck [CCDHB]
Sent: Tuesday, June 11, 2013 1:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Her-2 antibody
We here in wellington Hospital New Zealand having some problem with the
Her-2 ( Lieca, NCL-L-CBE-356 )
Top half of the slide ( control or Test tissue) are strongly positive but if we put the control tissue on the bottom half it is week to negative!
Does any one has the same problems with your Her-2, it is done on a Leica Bond (Max) III.
Much appreciated for any suggestions, also from Leica Technical specialists.
Wellington hospital, NZ
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