[Histonet] picric acid
Andrea.X.Dejager <@t> kp.org
Andrea.X.Dejager <@t> kp.org
Tue Jun 4 15:31:15 CDT 2013
Considering tissues were processed and are in paraffin there is no need to
panic, Picric acid was removed by solutions on the tissue processor.
Andrea De Jager, H.T. ASCP
Histology Manager, Regional Reference Lab
Kaiser Permanente - Colorado
Phone: 303-404-4152
Fax: 303-404-4161
email: ANDREA.X.DEJAGER <@t> KP.org
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From: histonet-request <@t> lists.utsouthwestern.edu
To: histonet <@t> lists.utsouthwestern.edu
Date: 06/04/2013 12:02 PM
Subject: Histonet Digest, Vol 115, Issue 4
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Today's Topics:
1. picric acid paranoia (Tyrone Genade)
2. Re: picric acid paranoia (Bryan Llewellyn)
3. RE: picric acid paranoia (Morken, Timothy)
4. Job Opening (Karen Vickers)
5. Highlands J Virus (Jan Shivers)
6. RE: picric acid paranoia (Smith, Allen)
7. Re: picric acid paranoia (Will Chappell)
----------------------------------------------------------------------
Message: 1
Date: Mon, 3 Jun 2013 20:30:08 +0200
From: Tyrone Genade <tgenade <@t> gmail.com>
Subject: [Histonet] picric acid paranoia
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAEYEE3kTbYxw=uc0sjt3Sx7=K9LjT0xTP9eunMWsgz__UtkgGw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello,
I am moving to the USA from sunny South Africa. I would like to bring my
wax blocks with me but the fish inside them were fixed with Bouin's fluid.
I'm worried the picric acid could draw the wrong sort of attention.
Courier
companies and US Customs (which never got back to me) haven't been able to
give me an answer if they are safe to travel. The blocks have sat under my
lab bench for 4 years without blowing up so I guess they are perfectly
safe. Anyone have an opinion on the issues or some advice on an expert (at
US customs?) to contact? I would probably ship them by surface post as it
just more cost effective.
Thanks
Tyrone Genade PhD
Department of Human Biology
University of Cape Town
------------------------------
Message: 2
Date: Mon, 03 Jun 2013 12:04:56 -0700
From: Bryan Llewellyn <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] picric acid paranoia
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <51ACE8D8.1080305 <@t> shaw.ca>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
I don't know what the US regulations for importing paraffin blocks are,
but Bouin fixed and paraffin processed tissues are perfectly safe. It is
picric acid and its simple salts which are dangerous when dried. Since
your tissues went through alcohol during processing, any free picric
acid will have been removed and only that attached to the proteins
remains. In fact, the paraffin wax itself is likely more of a hazard
since it is inflammable. I suspect they could be safely shipped by mail,
with appropriate declarations.
Bryan Llewellyn
Tyrone Genade wrote:
> Hello,
>
> I am moving to the USA from sunny South Africa. I would like to bring my
> wax blocks with me but the fish inside them were fixed with Bouin's
fluid.
> I'm worried the picric acid could draw the wrong sort of attention.
Courier
> companies and US Customs (which never got back to me) haven't been able
to
> give me an answer if they are safe to travel. The blocks have sat under
my
> lab bench for 4 years without blowing up so I guess they are perfectly
> safe. Anyone have an opinion on the issues or some advice on an expert
(at
> US customs?) to contact? I would probably ship them by surface post as
it
> just more cost effective.
>
> Thanks
>
> Tyrone Genade PhD
> Department of Human Biology
> University of Cape Town
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 3
Date: Mon, 3 Jun 2013 19:10:14 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: RE: [Histonet] picric acid paranoia
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <761E2B5697F795489C8710BCC72141FF0826F5 <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset=us-ascii
I agree with Bryan, the only dangerous form is anhydrous powder.
I'm thinking they might be more interested in having you declare these
blocks are not infectious...
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bryan
Llewellyn
Sent: Monday, June 03, 2013 12:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] picric acid paranoia
I don't know what the US regulations for importing paraffin blocks are,
but Bouin fixed and paraffin processed tissues are perfectly safe. It is
picric acid and its simple salts which are dangerous when dried. Since
your tissues went through alcohol during processing, any free picric acid
will have been removed and only that attached to the proteins remains. In
fact, the paraffin wax itself is likely more of a hazard since it is
inflammable. I suspect they could be safely shipped by mail, with
appropriate declarations.
Bryan Llewellyn
Tyrone Genade wrote:
> Hello,
>
> I am moving to the USA from sunny South Africa. I would like to bring
> my wax blocks with me but the fish inside them were fixed with Bouin's
fluid.
> I'm worried the picric acid could draw the wrong sort of attention.
> Courier companies and US Customs (which never got back to me) haven't
> been able to give me an answer if they are safe to travel. The blocks
> have sat under my lab bench for 4 years without blowing up so I guess
> they are perfectly safe. Anyone have an opinion on the issues or some
> advice on an expert (at US customs?) to contact? I would probably ship
> them by surface post as it just more cost effective.
>
> Thanks
>
> Tyrone Genade PhD
> Department of Human Biology
> University of Cape Town
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
_______________________________________________
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------------------------------
Message: 4
Date: Mon, 3 Jun 2013 14:18:08 -0500
From: "Karen Vickers" <kvickers <@t> westmoreland-derm.com>
Subject: [Histonet] Job Opening
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <004901ce608f$16026a90$42073fb0$@com>
Content-Type: text/plain; charset="us-ascii"
We have a job opening in our dermatopathology lab for a Histotechnician.
If
interested, please submit resume to:
Westmoreland Dermatology
P. O. Box 8695
Columbus, MS 39705
or email kvickers <@t> westmoreland-derm.com
Thank you,
Karen Vickers
Westmoreland Dermatology
Administrator
phone 662-243-2435
fax 662-328-7037
cell 662-425-3769
------------------------------
Message: 5
Date: Mon, 3 Jun 2013 15:30:04 -0500
From: Jan Shivers <shive003 <@t> umn.edu>
Subject: [Histonet] Highlands J Virus
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAEoC1q1dQb8AOGVfUpPni_yy7V6rMD54HO33eaZr4iyhe87grQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
(Asking for a colleague...)
Is there any lab out there currently doing IHC for Highlands J Virus? You
can message me privately.
Thanks in advance,
Jan Shivers
Senior Scientist
Univ. of Minnesota Veterinary Diagnostic Lab
shive003 <@t> umn.edu
------------------------------
Message: 6
Date: Tue, 4 Jun 2013 12:52:26 +0000
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] picric acid paranoia
To: "tgenade <@t> gmail.com" <tgenade <@t> gmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<93D1C752AF965C43AE4C84D6D45CFC2FDFF156 <@t> BL2PRD0710MB373.namprd07.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
Picric acid bound to collagen is not an explosion hazard. Even if it
were, the surrounding paraffin wax would cushion the picric acid to the
point of making it shockproof. Most of the picric acid in a fixative ends
up in the hazmat bottle rather than in the tissue. Thus even putting 50
or so blocks of tissue fixed in picric acid into a hot fire would create
less blast than a hearing aid battery.
Bulk picric acid, where there is no moderator between the crystals, is
another story.
- Allen A. Smith, Ph.D.
Barry University School of Podiatric Medicine
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tyrone
Genade
Sent: Monday, June 03, 2013 2:30 PM
To: histonet
Subject: [Histonet] picric acid paranoia
Hello,
I am moving to the USA from sunny South Africa. I would like to bring my
wax blocks with me but the fish inside them were fixed with Bouin's fluid.
I'm worried the picric acid could draw the wrong sort of attention.
Courier companies and US Customs (which never got back to me) haven't been
able to give me an answer if they are safe to travel. The blocks have sat
under my lab bench for 4 years without blowing up so I guess they are
perfectly safe. Anyone have an opinion on the issues or some advice on an
expert (at US customs?) to contact? I would probably ship them by surface
post as it just more cost effective.
Thanks
Tyrone Genade PhD
Department of Human Biology
University of Cape Town
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 7
Date: Tue, 4 Jun 2013 06:22:04 -0700
From: Will Chappell <chapcl <@t> yahoo.com>
Subject: Re: [Histonet] picric acid paranoia
To: "Smith, Allen" <asmith <@t> mail.barry.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "tgenade <@t> gmail.com"
<tgenade <@t> gmail.com>
Message-ID: <57AFF7C7-473B-4820-A3F4-6BDF70AEBD79 <@t> yahoo.com>
Content-Type: text/plain; charset=us-ascii
Ship them as you would any biological test samples. No problems here.
Sent from my iPhone
On Jun 4, 2013, at 5:52 AM, "Smith, Allen" <asmith <@t> mail.barry.edu> wrote:
> Picric acid bound to collagen is not an explosion hazard. Even if it
were, the surrounding paraffin wax would cushion the picric acid to the
point of making it shockproof. Most of the picric acid in a fixative ends
up in the hazmat bottle rather than in the tissue. Thus even putting 50
or so blocks of tissue fixed in picric acid into a hot fire would create
less blast than a hearing aid battery.
> Bulk picric acid, where there is no moderator between the crystals, is
another story.
> - Allen A. Smith, Ph.D.
> Barry University School of Podiatric Medicine
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tyrone
Genade
> Sent: Monday, June 03, 2013 2:30 PM
> To: histonet
> Subject: [Histonet] picric acid paranoia
>
> Hello,
>
> I am moving to the USA from sunny South Africa. I would like to bring my
wax blocks with me but the fish inside them were fixed with Bouin's fluid.
> I'm worried the picric acid could draw the wrong sort of attention.
Courier companies and US Customs (which never got back to me) haven't been
able to give me an answer if they are safe to travel. The blocks have sat
under my lab bench for 4 years without blowing up so I guess they are
perfectly safe. Anyone have an opinion on the issues or some advice on an
expert (at US customs?) to contact? I would probably ship them by surface
post as it just more cost effective.
>
> Thanks
>
> Tyrone Genade PhD
> Department of Human Biology
> University of Cape Town
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
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