[Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available?

[Thurby, Christina]

Thanks James and Liz!  I'll look into the Iba1, this is an antibody I have not looked at yet!

Christina

>-----Original Message-----
>From: James Watson [mailto:JWatson <@t> gnf.org]
>Sent: Tuesday, June 04, 2013 2:09 PM
>To: 'Elizabeth Chlipala'; Thurby, Christina;
>histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Anyone know if commercial antibodies to KCA-1
>and/or KCA-2 (Kupffer cell antibodies) are available?
>
>We are in the process of working up the Iba1 on mouse and it does stain
>the Kupffer cells, macrophages, and microglial
>
>James Watson HT  ASCP
>GNF  Genomics Institute of the Novartis Research Foundation
>Tel    858-332-4647
>Fax   858-812-1915
>jwatson <@t> gnf.org
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-
>bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala
>Sent: Tuesday, June 04, 2013 12:03 PM
>To: Thurby, Christina; histonet <@t> lists.utsouthwestern.edu
>Subject: RE: [Histonet] Anyone know if commercial antibodies to KCA-1
>and/or KCA-2 (Kupffer cell antibodies) are available?
>
>Christy
>
>This is not necessarily specific for kupffer cells it will stain other
>macrophages but F4/80 does stain Kupffer cells.  I guess I should ask
>which species, ED-1 will work in rat, the F4/80 in mouse and I have
>heard that Iba-1 stains macrophages in mice nicely that's a microglia
>marker, but we have not tried it on any other tissue than brain so far.
>
>Liz
>
>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier
>Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax
>(303) 682-9060 Cell (303) 881-0763 liz <@t> premierlab.com www.premierlab.com
>
>Ship to address:
>
>1567 Skyway Drive, Unit E
>Longmont, CO 80504
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-
>bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thurby, Christina
>Sent: Tuesday, June 04, 2013 12:22 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or
>KCA-2 (Kupffer cell antibodies) are available?
>
>Hi all,
>I have been searching for a Kupffer cell antibody.  I read a paper that
>KCA-1 and KCA-2 are two monoclonal antibodies that are specific for
>Kupffer cells (PMCID: PMC1877398) .  I am looking for a vendor that may
>have these antibodies available commercially.  Alternatively, if anyone
>knows of other antibodies that are specific for Kupffer cells that would
>also be helpful!
>Thanks!
>
>Christina Thurby
>Bristol Myers Squibb
>812-307-2093
>
>
>
>
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