[Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available?

[Elizabeth Chlipala]

Christy

This is not necessarily specific for kupffer cells it will stain other macrophages but F4/80 does stain Kupffer cells.  I guess I should ask which species, ED-1 will work in rat, the F4/80 in mouse and I have heard that Iba-1 stains macrophages in mice nicely that's a microglia marker, but we have not tried it on any other tissue than brain so far.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
Work (303) 682-3949
Fax (303) 682-9060
Cell (303) 881-0763
liz <@t> premierlab.com
www.premierlab.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thurby, Christina
Sent: Tuesday, June 04, 2013 12:22 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available?

Hi all,
I have been searching for a Kupffer cell antibody.  I read a paper that KCA-1 and KCA-2 are two monoclonal antibodies that are specific for Kupffer cells (PMCID: PMC1877398) .  I am looking for a vendor that may have these antibodies available commercially.  Alternatively, if anyone knows of other antibodies that are specific for Kupffer cells that would also be helpful!
Thanks!

Christina Thurby
Bristol Myers Squibb
812-307-2093




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