[Histonet] Losing tissue on IHC slides

[Benjamin]

So the patient tissue and control are on the same slide, and the control is staying on but patient tissue is falling off? Maybe dont bake the control tissue just let them air dry, the humidity in the slide drying oven might be messing with the super frost plus slides. Its just a coating on the slide of some sort, i know after the first dip they dont work again like the first time, so just air dry the controls and bake after patient tissue is picked up.

Sent from my iPhone

On Jul 26, 2013, at 5:09 PM, Deloris Carter <delsuec <@t> gmail.com> wrote:

> Hi all,
> I'm looking for some troubleshooting help.  In the last couple of months
> we've been having an increasing trend in losing tissue from our IHC
> slides.  We use a Ventana Benchmark XT.  PM is done on it quarterly.  The
> problem seems to be with the patient tissue, not the control tissue.  We
> use Fisherbrand Superfrost Plus slides, picking up the control by dipping
> the top end of the slide to avoid "double dipping".  We use tap water in
> the waterbaths which I know could cause an issue, but that's the way it's
> always been done here, so there's been no change to that part of the
> procedure. (We did try using DI water, but it didn't make any difference.
> Tissue still fell off of slides.) We do use DI water to mix the bulk
> reagents.The control slides may sometimes have been cut for a longer than
> recommended time, but that part of the procedure hasn't changed either,
> same process as always.  If we run separate slides for control and patient
> tissue, we get much better results, but still have some tissue loss.  I
> can't say it's all of our tests, but it is a steadily worsening problem.
> I'm going through tons of reagent, and I can't seem to nail down the
> problem.  Ventana rep said to try DI water, don't double dip, try adhesive
> slides (which didn't really make any difference that we could tell).  I
> would appreciate any suggestions
> Deloris Carter
> Shawnee Mission Medical Center
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