[Histonet] Spirochete IHC on H-Pylori infected Tissue ?

[Goins, Tresa]

Belma -

Whether or not the spirochete antibody would work with H. pylori depends entirely on the anti-spirochete antibody specificity.
We use an anti-chlamydia antibody that hits all Chlamydia sp.; others are species specific.

The data sheet or the WEB site for the spirochete antibody should provide that information.

Tresa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of a b
Sent: Wednesday, July 24, 2013 3:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Spirochete IHC on H-Pylori infected Tissue ?

Rene that sounded really rude, its a simple yes or no question. Some medical journals list H-Pylori as a "Spirochete"!
 that's why I asked in the first place... I wonder what your supervisor thinks of your knowledge,  especially when you spelled H-Pylori > " H-pilori" ??? hmm. -__-

On Sun, Jul 21, 2013 at 3:11 PM, Rene J Buesa <rjbuesa <@t> yahoo.com<mailto:rjbuesa <@t> yahoo.com>> wrote:

> You are overlooking the essential characteristic of IHC, namely,
> specificity.
> Ab to H-Pilory will react only with H-Pilory, and the same goes for
> Spirochete.
> You will be wasting all your reagents and, worse of everything, your
> supervisor will wonder about your knowledge.
> René J.
>
>   *From:* a b <zipper210 <@t> gmail.com<mailto:zipper210 <@t> gmail.com>>
> *To:* histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
> *Sent:* Saturday, July 20, 2013 10:09 PM
> *Subject:* [Histonet] Spirochete IHC on H-Pylori infected Tissue ?
>
> So here I am working my Saturday night, running an Immuno for Monday,
> only to open the fridge and find no more H-Pylori antibody cause we're
> out! : ( Can I substitute with Spirochete Antigen, and If so will the
> result will be as strong?
>
> As a standard procedure, I am doing a Warthin Starry on it also,
>
> Advice would be great. Thanks
>
> Belma Woodcraft ( Histology Lead )
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