[Histonet] Sections

[joelle weaver]

Agreed.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> Date: Tue, 23 Jul 2013 14:56:15 -0500
> From: jaylundgren <@t> gmail.com
> To: tony.auge <@t> gmail.com
> Subject: Re: [Histonet] Sections
> CC: histonet <@t> lists.utsouthwestern.edu; gmartin <@t> marshallmedical.org
> 
>     I've worked places where they've used all kinds of leveling methods,
> specifying number of microns between levels, etc.  I cut exactly whatever
> the pathologist wants me to cut.  I've traveled for 16 years now.  Think
> about some of the wacky crap I've seen.
>     A GOOD histotech, in my humble opinion, ( assuming a new, state of the
> art mirotome, new disposable blade, cold, rough cut, hydrated blocks)
> should be able to lay out a 21 section 3um ribbon of a GI bx longways on
> the water bath in one go.
>     Said histotech  would always  ignore the first and last sections of
> said 21 section ribbon (one is touching the waterbath, and the terminal one
> touched the forceps/stick/finger/etc) and pick up the 2nd/3rd, 12th/13th,
> and 19th/20th sections. This is 3 levels.
>      A BAD histotech will lay out a six section ribbon, take six sections
> (and a floater),  and call it 3 levels.
>      Make absolutely sure that you are rough cutting into the block
> adequately to provide a full face section.  Most of your recuts will come
> from under-faced blocks.   But try to cut and float nice LOOOOOOONG ribbons
> to pick up your sections from and you will get nicer sections and less
> recuts.  What takes time cutting (traditional) levels is the refacing and
> recooling/rehydration of the block.
>  If you can get true levels off of one long ribbon (not 6 adjacent
> sections), and not increase recuts as a result, you win twice. So does the
> patient. :)
> 
>                                      Sincerely,
> 
>                                            Jay A. Lundgren, M.S., HTL (ASCP)
> 
> 
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> 
> 
> On Tue, Jul 23, 2013 at 2:04 PM, Tony Auge <tony.auge <@t> gmail.com> wrote:
> 
> > We cut the way your Pathologist is requesting at my lab, it's simpler and
> > faster then the way you are currently cutting. I was used to cutting the
> > way you described but this way is much easier. Check with your other
> > Pathologists that they will be ok with the switch. I have certain
> > Pathologist that will order more deepers because they want to see more of
> > the block. It will speed up cutting in the first place but may take more
> > time to cut deepers later in the day.
> > --
> >
> > Tony Auge HTL (ASCP) QIHC
> > Cell: (651) 373-4768
> > Email: tony.auge <@t> gmail.com
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