[Histonet] RE: Sections

[joelle weaver]

I have heard of and performed both methods. The variables seem to be with a ribbon, that if some fold, wrinkle, or fall off, you basically have more sections of the same depth on one slide. That being said, if that level is bad or  superficial, you will get recuts/deepers. If they are only needing one depth into the tissue for initial diagnosis or are worried about conserving tissue, then a ribbon might work. If they want progression, then you need more levels, one slide or many. Without going full rotations there wouldn't be much progression in depth in a single ribbon, especially at the thickness one would normally cut biopsy sections. Personally I like levels, one somewhat superficial section that is still a complete outline, one slightly deeper and one fully into the specimen without cutting anything away. Personally levels not only save recuts to me, but provide only one slide for labeling ( less labeling error opportunities), higher staining throughput/TAT/cases, less storage, less slides...you get the idea.  of course ultimately it is up to the Pathologist and sometimes others, but was there some reason given for the change in preference? 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: flnails <@t> texaschildrens.org
> To: Joyce.Weems <@t> emoryhealthcare.org; gmartin <@t> marshallmedical.org; histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 23 Jul 2013 19:46:52 +0000
> CC: 
> Subject: [Histonet] RE: Sections
> 
> I have heard of both methods, currently we put double ribbons on one slide.
> The only time we have repeats is if the initial cut is superficial. 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
> Sent: Tuesday, July 23, 2013 2:22 PM
> To: 'Martin, Gary'; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] RE: Sections
> 
> If placing more than 1 section of the same level on a slide, I've always cut three slides. I've never had a pathologist who only wanted to see one level.
> 
> Joyce Weems
> Pathology Manager
> 678-843-7376 Phone
> 678-843-7831 Fax
> joyce.weems <@t> emoryhealthcare.org
> 
> 
> 
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Gary
> Sent: Tuesday, July 23, 2013 2:47 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Sections
> 
> One of our pathologist has requested a change in the way we cut GI specimens.  We presently put three levels on one slide. Exp.  we face the block, cut a ribbon and take one section of that ribbon and catch on the slide. We then progress into the block and repeat the process until we have three levels on the slide.  The new suggestion is to simply face the block, create a ribbon, and take three to four section of that ribbon and that's it no more progression into the block.  The thinking is that the Pathologist will see what they need to in those levels, and if not they will call for "deepers". I have never cut like this and just wanted to hear some thoughts on this method.
> 
> Thanks
> 
> Gary
> 
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