[Histonet] RE: Histonet Digest, Vol 116, Issue 20

[Scott A. Ely]

We decalcify bone marrow core biopsies in EDTA with HCL, for 1 hour.  This works very well for us.

However, we have found that the HCL makes it impossible to do in situ hybridization with some probes (other probes work OK).

Do any of you do ISH on bone marrow core biopsies?  Does it work well with all needed probes, especially kappa and lambda?  If so, how do you decalcify?

Thank you.

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Scott Ely, MD MPH
Associate Director, Hematopathology Fellowship Program
Section of Hematopathology
Department of Pathology
Weill Medical College of Cornell University
New York Presbyterian Hospital
Room:  Starr 715
525 E. 68th Street
New York, NY 10065
PH:  212-746-2442
FAX: 212-746-2009
http://www.cornellphysicians.com/scottely/

Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by  Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message.
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From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Friday, July 19, 2013 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 116, Issue 20

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Today's Topics:

   1. Re: histology humor on youtube (susanbachus <@t> verizon.net)
   2. Re: Carnoy solution (Bob Richmond)


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Message: 1
Date: Fri, 19 Jul 2013 12:51:58 -0400
From: <susanbachus <@t> verizon.net>
Subject: Re: [Histonet] histology humor on youtube
To: "Emily Sours" <talulahgosh <@t> gmail.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1C89DD2B9F0B4B2AA2B6FAE610672E47 <@t> UserPC>
Content-Type: text/plain; format=flowed; charset=iso-8859-1;
        reply-type=original

Sorry, I don't even know what a farker is!   This was shared on a
veterinarian's FB page!  I almost sent the Lady Science one, which I think
is hysterical, but I was afraid it wasn't relevant enough to histology per
se (all biochemistry).    But I'm glad someone else did.   Well, maybe these
will give a creative histologist some ideas...

-----Original Message-----
From: Emily Sours
Sent: Friday, July 19, 2013 12:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] histology humor on youtube

Hey, I just found that today too!
Are you a farker??

I almost sent it to the list, but then decided someone had already done so
and I missed it.
I heard that the university is upset because it's gone viral, something
about inappropriate behavior in the lab.
NO HAVING FUN, EVER!!
:)


Emily


"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Fri, Jul 19, 2013 at 10:56 AM, <susanbachus <@t> verizon.net> wrote:

> I'm afraid I don't have the original query any more, but I just stumbled
> onto this video from a Pathology lab, which does include some histology.
> Be
> forewarned that there's some profanity. https://www.youtube.com/watch?**
> v=ZL7a3_JRWQY <https://www.youtube.com/watch?v=ZL7a3_JRWQY>
>
>
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Message: 2
Date: Fri, 19 Jul 2013 12:56:55 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Carnoy solution
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CAOKsRH76NcTepi5Mru8HPnMRN3r9g=NVd9SaYuUsMojobcS=xQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Carnoy's fixative contains chloroform, and that's enough reason not to use
it. In addition to its toxicity, chloroform dissolves methacrylate plastic,
as I found out the hard way once when I tried to fix a specimen in Carnoy's
fixative in a methacrylate container because I had nothing else.

I've seen Carnoy's fixative used as a "disclosing fixative" for finding
mesenteric lymph nodes in colectomy specimens. There's no excuse for this.
There are a number of proprietary fixatives (such as Surgipath's O-Fix) for
this purpose, or if you're allowed to brew your own, I use Davidson's
fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde
[NOT 10% NBF], 1 part glacial acetic acid.)

Bob Richmond
Samurai Pathologist
Maryville TN


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End of Histonet Digest, Vol 116, Issue 20
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