[Histonet] RE: Carnoys solution

[Tony Henwood (SCHN)]

Hi Allison,

It will depend on what you are using the Carnoy's for. 
If it is for the removal of blood from smeared cytology preps then Clarke's fluid, a 3:1 mixture of ethanol and acetic acid, works very well.

For fixation of tissue samples prior to processing to wax then you probably need to use the original Carnoy's recipe, though Meier's Fixative (Glacial acetic acid 25ml + 95% ethanol 475ml), with less acetic acid has been used in the past.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Friday, 19 July 2013 4:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Carnoys solution

Hello to all in histoland.  Does anyone have a carnoys recipe that does not have call for chloroform?  I had a recipe for one, but now I can't find it.
Any and all help will be appreciated.



Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287

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