AW: [Histonet] Eosin Staining Issues

[Gudrun Lang]

What about section-thickness? Do you have a tech, who cuts very thin?
I have sometimes this problem, that the staining looks very faint because
the slide is just too thin.

Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von kristen
arvidson
Gesendet: Freitag, 12. Juli 2013 19:25
An: histonet
Betreff: [Histonet] Eosin Staining Issues

Hello All!
 
I am having some bizzare staining issues.  The eosin seems to be washing
out??  The slides look "sun bleached" according to the Pathologists.  The
problem is that it is only happening on occasion.   One rack stains fine and
the next one has a problem.   It even appears to be  happening within cases
staining in the same rack.  My first though is tap water ph.  We don't
monitor that, haven't needed to,  we've never had a problem like this
before.  Thoughts??  Thanks _______________________________________________
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