[Histonet] RE: tol blue and mast cells

[Elizabeth Chlipala]

Richard

We use 0.04% T. Blue in an acetate buffer that is pH'd to 4.0.  Stain for about 5 minutes, rinse in water, let air dry and then coverslip in xylene.  You will loose the metachromatic staining if you run the slides through alcohol.  I have an SOP if you would like to see one.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz <@t> premierlab.com

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Longmont, CO 80504
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. [ree3 <@t> leicester.ac.uk]
Sent: Friday, July 12, 2013 9:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tol blue and mast cells

Hi  All, your  thoughts on the  best toluidine  blue method  for  mast cells?, many  thanks.


                             Richard  Edwards

                                       University of Leicester

                                              U.K.

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