[Histonet] RE: Histonet Digest, Vol 116, Issue 1
Jennifer Garrigan
JGarrigan <@t> cbiolabs.com
Tue Jul 2 14:32:25 CDT 2013
Re: H&E Staining
We do not have a high volume of rat tissues and thus stain by hand. Generally we have increased the time in hematoxylin and eosin slightly because the tissue is so dense, even if cut at the same thickness as mouse tissue. We do long term storage in 70% EtOH and have not had any problems with staining after that.
Hope this may help
Jennifer
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Sent: Tuesday, July 02, 2013 1:06 PM
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Subject: Histonet Digest, Vol 116, Issue 1
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Today's Topics:
1. renin IHC (Amy Lee)
2. specimen tracking systems (Stacy McLaughlin)
3. Immunohistochemistry TAT (Karlisch, Patricia)
4. DAB Testing (Lori Harris)
5. If going to holiday/vacation, or to unsubscribe (Lee & Peggy Wenk)
6. AW: [Histonet] Immunohistochemistry TAT (Gudrun Lang)
7. RE: Immunohistochemistry TAT (Sebree Linda A)
8. H&E staining question (Connolly, Brett M)
9. RE: H&E staining question - some clarification (Connolly, Brett M)
10. Re: H&E staining question (Colleen Forster)
11. RE: H&E staining question (Bea DeBrosse-Serra)
12. IHC Elastin stain (Sebree Linda A)
13. RE: IHC Elastin stain (Houston, Ronald)
14. RE: DAB Testing (Cindy Pyse)
----------------------------------------------------------------------
Message: 1
Date: Mon, 1 Jul 2013 11:05:50 -0700 (PDT)
From: Amy Lee <amylee779 <@t> yahoo.com>
Subject: [Histonet] renin IHC
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1372701950.60879.YahooMailNeo <@t> web126006.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
I am looking for a renin antibody for IHC on FFPE rodent tissue. I searched around and couldn't?decide which one I should try.?Could anybody recommend one?
?
Thanks in advance.
?
Amy
------------------------------
Message: 2
Date: Mon, 1 Jul 2013 18:38:30 +0000
From: Stacy McLaughlin <Stacy_McLaughlin <@t> cooley-dickinson.org>
Subject: [Histonet] specimen tracking systems
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<BED59CE76B971D45A9EF1240DE30F5E9134EB4 <@t> CDHMAIL02.cooley-dickinson.org>
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Hello Histoland,
Wondering how many of you use specimen tracking systems (bar-coded) and what type of system you use.
Pros-cons of each system?
Thanks,
Stacy
------------------------------
Message: 3
Date: Mon, 1 Jul 2013 21:35:32 +0000
From: "Karlisch, Patricia" <pkarlisch <@t> hmc.psu.edu>
Subject: [Histonet] Immunohistochemistry TAT
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
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Histonetters,
I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information.
* We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls.
* The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight.
* The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm.
Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight.
The following tasks makes the 3:30- 4pm deadline difficult:
* Searching for the blocks
* Cutting the blocks (40-50) onto control slides + negative control
* One hour in the oven to dry (Can we use 30minutes?)
* Attaching labels to 150 slides and setting up instruments
* One microtome
* 2 techs/ some of the time
Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process.
Thank you,
Pat Karlisch, Supervisor
pkarlisch <@t> hmc.psu.edu<mailto:pkarlisch <@t> hmc.psu.edu>
Tel 717-531-6072
------------------------------
Message: 4
Date: Mon, 1 Jul 2013 14:45:27 -0700
From: Lori Harris <lharris <@t> samhealth.org>
Subject: [Histonet] DAB Testing
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<450EDC37E404D142AF67D7314C954C8A3C7BCA943A <@t> SHSMAILVI01.int.samhealth.net>
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Hello All!
Can someone recommend a company that does DAB waste testing? I would like to get our waste tested from our Ventana Ultra. Thanks.
Lori A. Harris, HT (ASCP)
541-768-6078
Histology Section Lead
GSRMC Pathology Lab
3600 NW Samaritan Drive
Corvallis, OR 97330
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Message: 5
Date: Tue, 2 Jul 2013 05:55:03 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: [Histonet] If going to holiday/vacation, or to unsubscribe
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
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If you are going to vacation/holiday, please unsubscribe from Histonet. We don???t want a zillion messages saying ???I am out of the office.???
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Thank you.
Peggy A. Wenk, HTL(ASCP)SLS
------------------------------
Message: 6
Date: Tue, 2 Jul 2013 13:43:36 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] Immunohistochemistry TAT
To: "'Karlisch, Patricia'" <pkarlisch <@t> hmc.psu.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <001301ce7719$6459fd70$2d0df850$@gmx.at>
Content-Type: text/plain; charset="iso-8859-1"
Dear Pat,
here some inputs. If usefull, you have to decide for yourself.
Ad controls: We use for most of our antibodies an "universal-control". This is put together with tiny pieces of normal tonsil, appendix, skin, liver and pancreas. If you are interested, look at the website of NordiQC.
Some labs prefer to cut the controls in advance. This can also save time, but the controls shouldn't get older than a week.
Ad speed: we cut the urgent cases in advance besides the HE with 6 slides per block. These are needle biopsies from breast, liver, kidney, .... etc.; the pathologists only have to order a panel.
Ad drying: we often skip the drying in the oven at all. But it is important to get rid of the water under the sections. (Besides: drying at high temp for longer period can alter stainability.) Ad LIS: we also have no LIS, but a light-version of it. We use a WORD-document as form. The pathologists can mark the wished antibodies and print the sheet directly onto our printer in the lab.
Ad organisation: isn't it possible with 4 instruments to keep the same antibodies on each instrument? For example: one machine is the breast-machine, one for lymphomas, etc. If this is possible, you can add a "machine-code" on the labels and only look at this code while filling the machines.
We work only with one Ultra. First technician comes at 6.30 and starts the first run until 7.00-7.30. Second run is started at 10.30-11.00 and the run over night is started around 15.00. The most slides have to been cut at afternoon. And if there are more than 30, they go into the morning run.
This gives for the urgent biopsies an IHC-TAT about 5-6 hours (from HE delivering to IHC delivering). Most other cases are ready over night (HE delivering till 12.30 and IHC-ordering-deadline at 14.30).
Hope this helps
Gudrun Lang
Ltd. BMA
Histolabor Akh Linz, Austria
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Karlisch, Patricia
Gesendet: Montag, 01. Juli 2013 23:36
An: 'histonet <@t> lists.utsouthwestern.edu'
Betreff: [Histonet] Immunohistochemistry TAT
Histonetters,
I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information.
* We have a busy Immunohistochemistry lab and the volume of
requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for
>150- 200 IHC tests twice a day, not including negative and positive
>(same
slide) controls.
* The techs pull an AM log at 6am and another at 1pm. The 1pm log
will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight.
* The 6am log requires that one tech start cutting each block for
multiple IHC stains. (A search for blocks and the necessary control slide
occurs by the same person). The slides are kept in a 60 degree oven for
about 60 minutes. Most of these slides will be ready by 2-3:30pm.
Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3
Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need
to be cut; sit in an oven and then get labeled and placed on the Ventana's.
All longer stains such as ISH would be run overnight.
The following tasks makes the 3:30- 4pm deadline difficult:
* Searching for the blocks
* Cutting the blocks (40-50) onto control slides + negative control
* One hour in the oven to dry (Can we use 30minutes?)
* Attaching labels to 150 slides and setting up instruments
* One microtome
* 2 techs/ some of the time
Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process.
Thank you,
Pat Karlisch, Supervisor
pkarlisch <@t> hmc.psu.edu<mailto:pkarlisch <@t> hmc.psu.edu>
Tel 717-531-6072
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 2 Jul 2013 12:41:37 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: [Histonet] RE: Immunohistochemistry TAT
To: "'Karlisch, Patricia'" <pkarlisch <@t> hmc.psu.edu>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF0505C7 <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Pat,
Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some "Stay On" in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves searching for blocks. See if there is a way to have them organized immediately after sectioning the H&Es so they are quicker to find...we are struggling with this.
My 2 cents; good luck.
Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia
Sent: Monday, July 01, 2013 4:36 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Immunohistochemistry TAT
Histonetters,
I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information.
* We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for >150- 200 IHC tests twice a day, not including negative and positive (same slide) controls.
* The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight.
* The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm.
Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight.
The following tasks makes the 3:30- 4pm deadline difficult:
* Searching for the blocks
* Cutting the blocks (40-50) onto control slides + negative control
* One hour in the oven to dry (Can we use 30minutes?)
* Attaching labels to 150 slides and setting up instruments
* One microtome
* 2 techs/ some of the time
Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process.
Thank you,
Pat Karlisch, Supervisor
pkarlisch <@t> hmc.psu.edu<mailto:pkarlisch <@t> hmc.psu.edu>
Tel 717-531-6072
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 2 Jul 2013 09:07:42 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] H&E staining question
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C01C35B84DCDCE49BC60867E87F1C8FE6F50B67D58 <@t> USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"
Histonetters -
We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E.
The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study.
I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH.
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
------------------------------
Message: 9
Date: Tue, 2 Jul 2013 10:21:05 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: [Histonet] RE: H&E staining question - some clarification
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<C01C35B84DCDCE49BC60867E87F1C8FE6F50B67E34 <@t> USCTMXP51015.merck.com>
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I forgot to mention that the H&E was performed in another lab at our facility...most likely on an autostainer. We just did the IHC studies and I have always used 70% ETOH for tissue storage ... never heard of this staining phenomenon happening before.
Brett
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M
Sent: Tuesday, July 02, 2013 9:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E staining question
Histonetters -
We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E.
The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study.
I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH.
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
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------------------------------
Message: 10
Date: Tue, 02 Jul 2013 09:50:02 -0500
From: Colleen Forster <cforster <@t> umn.edu>
Subject: Re: [Histonet] H&E staining question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <51D2E89A.2070509 <@t> umn.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
I am interested in the answer as I use this same technique on my rodent samples and have not had this issue.
On 7/2/2013 8:07 AM, Connolly, Brett M wro
> Histonetters -
>
> We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E.
>
> The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study.
>
> I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH.
>
> Thanks,
> Brett
>
> Brett M. Connolly, Ph.D.
> Principal Scientist, Imaging Dept.
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_connolly <@t> merck.com
> T- 215-652-2501
> F- 215-993-6803
>
>
>
>
>
>
> Notice: This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station,
> New Jersey, USA 08889), and/or its affiliates Direct contact information
> for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from
> your system.
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Tue, 2 Jul 2013 08:18:30 -0700
From: Bea DeBrosse-Serra <BDeBrosse-Serra <@t> isisph.com>
Subject: [Histonet] RE: H&E staining question
To: "'Connolly, Brett M'" <brett_connolly <@t> merck.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<493CAA64F203E14E8823737B9EE0E25F09488DD8A6 <@t> EXCHMB01.isis.local>
Content-Type: text/plain; charset="us-ascii"
Hi Brett,
That the H&E staining looks faded after storage in 70% seems very unlikely. We do this with rat and mouse tissues all the time. Did the other lab use a different Hematoxylin, Eosin, or used a different staining protocol on the autostainer?
Bea
Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M
Sent: Tuesday, July 02, 2013 6:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E staining question
Histonetters -
We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs. Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E.
The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study.
I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results? Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH.
Thanks,
Brett
Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803
Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
_______________________________________________
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------------------------------
Message: 12
Date: Tue, 2 Jul 2013 16:00:21 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: [Histonet] IHC Elastin stain
To: "Histonet (Histonet <@t> lists.utsouthwestern.edu)"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF0506A0 <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Do any of you do this? Do any referral labs offer this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
------------------------------
Message: 13
Date: Tue, 2 Jul 2013 16:05:12 +0000
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] RE: IHC Elastin stain
To: Sebree Linda A <LSebree <@t> uwhealth.org>, "Histonet
(Histonet <@t> lists.utsouthwestern.edu)"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E5D0CD2352E46545A0C0EBE308CCCE5301C49BAF <@t> L1PERDWXMB01.childrensroot.net>
Content-Type: text/plain; charset="us-ascii"
Linda,
we do it here
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org
www.NationwideChildrens.org
"One person with passion is better than forty people merely interested."
~ E.M. Forster
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Tuesday, July 02, 2013 12:00 PM
To: Histonet (Histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] IHC Elastin stain
Do any of you do this? Do any referral labs offer this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Tue, 2 Jul 2013 12:08:17 -0400
From: "Cindy Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] DAB Testing
To: "'Lori Harris'" <lharris <@t> samhealth.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002c01ce773e$5db454a0$191cfde0$@x-celllab.com>
Content-Type: text/plain; charset="us-ascii"
I would be interested in this information as well.
Cindy
Cindy Pyse CLT, HT(ASCP)
Laboratory Manager
X-Cell Laboratories of WNY
20 Northpointe Parkway Ste 100
Amherst, NY 14228
716-250-9235 Ext. 232
cpyse <@t> x-celllab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lori Harris
Sent: Monday, July 01, 2013 5:45 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] DAB Testing
Hello All!
Can someone recommend a company that does DAB waste testing? I would like to
get our waste tested from our Ventana Ultra. Thanks.
Lori A. Harris, HT (ASCP)
541-768-6078
Histology Section Lead
GSRMC Pathology Lab
3600 NW Samaritan Drive
Corvallis, OR 97330
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End of Histonet Digest, Vol 116, Issue 1
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