[Histonet] H&E staining question

Colleen Forster cforster <@t> umn.edu
Tue Jul 2 09:50:02 CDT 2013

I am interested in the answer as I use this same technique on my rodent 
samples and have not had this issue.

On 7/2/2013 8:07 AM, Connolly, Brett M wro
> Histonetters -
> We had some rat tissues that were removed at necropsy and immediately fixed in formalin for 48 hrs.  Since the tissues could not be processed after the 48 hr fixation they were transferred to 70% ETOH for about a week, then processed and stained with H&E.
> The H&E staining looks faded/washed out when compared to previous rat tissue that was fixed for 48 hr and then processed and stained without the extended time in 70% ETOH. The reason for limiting the fixation to 48 hr in the more recent study was that we were doing some IHC stains and wanted to keep the same fixation time as the previous study.
> I have extra unstained slides left over - any suggestions on how to get some better, crisper H&E results?  Has anyone observed the washed out H&E appearance from tissues stored in 70% ETOH.
> Thanks,
> Brett
> Brett M. Connolly, Ph.D.
> Principal Scientist, Imaging Dept.
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_connolly <@t> merck.com
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