[Histonet] Question about sectioning brain tissue at 10-15 microns

Thurby, Christina christina.thurby <@t> bms.com
Thu Jan 31 11:10:04 CST 2013

Can anyone with experience in cutting thick FFPE brain sections please give advice on how to get a thick brain section without it curling up before it gets to the waterbath?  We are doing okay at cutting these at 10 microns, but are trying to get some 12 micron sections and the section just curls as it is being cut on the microtome.    We use Paraplast - just in case anyone needs to know.  I did read that having a lower temp melting point paraffin sometimes helps with this issue.  Any other thoughts from folks that routinely do this?
Christina Thurby
Bristol Myers Squibb

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