[Histonet] Question about sectioning brain tissue at 10-15 microns
christina.thurby <@t> bms.com
Thu Jan 31 11:10:04 CST 2013
Can anyone with experience in cutting thick FFPE brain sections please give advice on how to get a thick brain section without it curling up before it gets to the waterbath? We are doing okay at cutting these at 10 microns, but are trying to get some 12 micron sections and the section just curls as it is being cut on the microtome. We use Paraplast - just in case anyone needs to know. I did read that having a lower temp melting point paraffin sometimes helps with this issue. Any other thoughts from folks that routinely do this?
Bristol Myers Squibb
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