[Histonet] LFB Lithium Carbonate Differentiator alternative

Tim Wheelock twheelock <@t> mclean.harvard.edu
Wed Jan 30 10:39:44 CST 2013

Hi Cheryl:

Yes there is:
1% Hydroquinone
5% Sodium Sulfite
in Distilled water

This differentiator, which I use in my  lab, acts very quickly.
For a 5 micron paraffin section, I dip it only twice, quickly, into the 
differentiator, and then immediately, into 4 changes of tap water, then 
a staining dish with tap water.

Hope this helps,


Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA

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