FW: [Histonet] Re: Thrombin for cell block preparations

Hannen, Valerie Valerie.Hannen <@t> parrishmed.com
Tue Jan 29 04:48:08 CST 2013


-----Original Message-----
From: Hannen, Valerie 
Sent: Tuesday, January 29, 2013 5:46 AM
To: 'Bob Richmond'
Subject: RE: [Histonet] Re: Thrombin for cell block preparations

We get some Thromboplastin from the Hemo. Deparment and get Plasma from Blood Bank, when we need to make a cell block we add an equal amount of drops from both and let the specimen clot up.
Hope this helps!!


Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Monday, January 28, 2013 5:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Thrombin for cell block preparations

Laurie Best, Histology Supervisor at Sunrise Medical Labs, Hicksville NY asks:

>>I am trying to purchase thrombin for cell block preparation, and have 
>>been running into dead ends. Can someone give me their vendor 

I know about using thrombin to clot plasma to form cytopathologists'
cell blocks, but have never seen it done, and don't know a source.

In the many labs I've worked in, I've found that cell block methods and results vary greatly from lab to lab, from labs that don't do them at all, to labs that do them routinely on pleural and peritoneal fluids with uniformly good results. If I were setting up a procedure, I'd probably try the commercial agar preparation Histogel.

Cell blocks are a necessary cytologic preparation, the more so as the use of immunohistochemistry increases.

Bob Richmond
Samurai Pathologist
Maryville TN

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