[Histonet] RE: Histonet Digest, Vol 110, Issue 22

Riesen, Rebecca Rebecca.Riesen <@t> hma.com
Fri Jan 25 13:21:49 CST 2013

My Doc's don't like the "just ignore it" answer.  They want me to "just fix it"!  I repeat/ I tweak/ and we move on until the next one pops up and then we start all over again with the same discussion.  I would really appreciate some kind of "official" answer from some vendors development teams.  We (histologists) really deserve an answer. 

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Subject: Histonet Digest, Vol 110, Issue 22

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Today's Topics:

   1. Re: Helicobacter pylori immunocytochemistry -	cytoplasmatic
      staining (Bob Richmond)


Message: 1
Date: Wed, 16 Jan 2013 11:18:30 -0500
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Helicobacter pylori immunocytochemistry -
	cytoplasmatic staining
To: histonet <@t> lists.utsouthwestern.edu
	<CAOKsRH5NJUEQRdXgvgvC-ewAJyfz_g9g_w6fnFmFJdpG_NKNaw <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

I quite often see faint cytoplasmic staining in immunostains for Helicobacter. You're supposed to ignore it. It's often present in the negative control slide - one of the few reasons I ever look at a negative control slide.

These days I get my IHC for Helicobacter from whatever Genzyme is called this week. I'm noticing considerably less cytoplasmic staining than I've usually seen before. (I have no commercial interest here.)

Bob Richmond
Samurai Pathologist
Maryville TN


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