[Histonet] RE: Eosin intensity

[Tony Henwood (SCHN)]

Ceri,

There are several ways to darken the eosin counterstain:
1.	Stain longer in eosin, though with some eosins including eosin Y, this might not necessarily darken the staining since the eosin Y is a yellow red, eosin B seems to be more bluish red
2.	Under-differentiate the haematoxylin, though this can result in overly stained nuclei (especially those of plasma cells).
3.	Include a darker red dye in your eosin stain eg Erythrosine or even phloxine (not quite as dark).



Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ceri Allen
Sent: Wednesday, 23 January 2013 8:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosin intensity

Dear All,

Please can I ask what everyone uses as a standard Eosin recipe? The new pathologist in our department has asked for the stain to be darker, 'like the hospital does it'. I work on the research side of a vet school, so I'm not sure what histology departments in hospitals normally use.

Many thanks

Ceri

Research Technician
School of Veterinary Medicine and Science University of Nottingham




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