[Histonet] P21 mouse brain

[Geoff McAuliffe]

On 1/23/2013 11:10 AM, Papke, Louisa M. wrote:
> Has anyone worked with P21 mouse brain?  Such as frozen or paraffin sectioning?  Right now after perfusion and paraffin embedding, the brain literally falls apart when picking it up to embed.  Any ideas or hints available would be welcome.
>
> Thank you in advanced,
> Louisa M Papke
>
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>
Perfusion with what? Fixation for how long? What is your processing 
schedule?

Geoff

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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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