[Histonet] Which fixative for eyes

[tahseen <@t> brain.net.pk]

Dear Pedro Louro
We are using Alcoholic formalin fixative for ocular (Eye) specimens,
Reference Tech Sample (ASAP) HT-2(1995), since last 19 year without facing
any problem.

Composition:
Ethanol absolute 90 ml
Formaldehyde con 10 ml
Fix for 24 hours

Thanks,
Muhammad Tahseen
Histology Supervisor
SKMCH&RC Lahore
Pakistan

> Hello to the eye experts,
>
>
>
> I am currently working on a large animal (LA) eye project which will
> give me the flexibility of experimenting with different fixatives.
>
> I am currently using Modified Davidson's solution (48 hours) followed by
> a 10% NBF solution prior to trimming the eyes, embedding , microtomy and
> H&E staining.
>
>
>
> After literature research, I found that different labs are using:
>
>
>
> Modified Davidson's solution
>
> Davidson's solution
>
> 10% NBF
>
> Gluteraldehyde
>
> Some places inject the eye with fixative before submerging the eye
> itself.
>
>
>
> I wanted to get some ideas / preferences from the experts out there so I
> can cover my entire basis before finalizing my conclusions.
>
>
>
> Thanks in advance to all,
>
>
>
> Pedro Louro
>
> Group Leader Histology / IHC
>
>
>
>
>
>
>
>
> ********************************************************************************************
> Our Values:  Excellence, Drive, Ownership, Challenge, Teamwork, Respect
> ********************************************************************************************
>
> LEGAL NOTICE
> This message is confidential and contains information which may be legally
> privileged. It is intended for the stated addressee(s) only. Access to
> this email by anyone else is unauthorised. If you are not the intended
> addressee, any disclosure or copying of the contents of this email or any
> action taken (or not taken) in reliance on it is unauthorised and is
> unlawful. If you are not the addressee, please inform the sender
> immediately and destroy the original message.
> The data in this email will have been screened for the presence of
> computer viruses known by the Company at the time the email was produced.
> The Company cannot guarantee, however, that the email or any attachments
> are virus free. Delivery of the email will be at the Client's risk.
> Registered in England No. 1815730
> VAT Reg. No. GB425507072
> Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire
> PE28 4HS
> **********************************************************************
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>