[Histonet] Which fixative for eyes

tahseen <@t> brain.net.pk tahseen <@t> brain.net.pk
Thu Jan 17 22:06:22 CST 2013


Dear Pedro Louro
We are using Alcoholic formalin fixative for ocular (Eye) specimens,
Reference Tech Sample (ASAP) HT-2(1995), since last 19 year without facing
any problem.

Composition:
Ethanol absolute 90 ml
Formaldehyde con 10 ml
Fix for 24 hours

Thanks,
Muhammad Tahseen
Histology Supervisor
SKMCH&RC Lahore
Pakistan

> Hello to the eye experts,
>
>
>
> I am currently working on a large animal (LA) eye project which will
> give me the flexibility of experimenting with different fixatives.
>
> I am currently using Modified Davidson's solution (48 hours) followed by
> a 10% NBF solution prior to trimming the eyes, embedding , microtomy and
> H&E staining.
>
>
>
> After literature research, I found that different labs are using:
>
>
>
> Modified Davidson's solution
>
> Davidson's solution
>
> 10% NBF
>
> Gluteraldehyde
>
> Some places inject the eye with fixative before submerging the eye
> itself.
>
>
>
> I wanted to get some ideas / preferences from the experts out there so I
> can cover my entire basis before finalizing my conclusions.
>
>
>
> Thanks in advance to all,
>
>
>
> Pedro Louro
>
> Group Leader Histology / IHC
>
>
>
>
>
>
>
>
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