[Histonet] RE: Fixing and sectioning mouse eye

Elizabeth Chlipala liz <@t> premierlab.com
Thu Jan 17 09:50:23 CST 2013


It depends on what portion of the eye you are looking at, its this just general tox or a study that is specific for retinopathy


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell

Ship to address:

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Garrigan
Sent: Thursday, January 17, 2013 8:45 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Fixing and sectioning mouse eye

Hi All,
It was recently requested that I section mouse eyes. They were collected in 10% NBF, and then placed in PBS after 48 hours for storage. I cannot find much online with regard to how to proceed. Any suggestions?
Are there any special fixation and processing protocols you use?
Any information that you could provide would be extremely helpful.

Thank you
Jennifer Garrigan
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